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Site‐saturation Mutagenesis: A Powerful Tool for Structure‐Based Design of Combinatorial Mutation Libraries
This unit describes a method for site‐saturation mutagenesis (SSM) using PCR amplification with degenerate synthetic oligonucleotides as primers, a powerful approach in protein engineering to characterize structure‐function relationships, as well as to create improved protein variants. Expand
Plant glutathione transferase-mediated stress tolerance: functions and biotechnological applications
The biotechnological applications of GSTs towards developing plants that are resistant to biotic and abiotic stresses are reviewed and it is proposed that future GST engineering efforts through rational and combinatorial approaches, would lead to the design of improved isoenzymes with purpose-designed catalytic activities and novel functional properties. Expand
Catalytic features and crystal structure of a tau class glutathione transferase from Glycine max specifically upregulated in response to soybean mosaic virus infections.
The results of the present study provide new information into GST diversity and give further insights into the complex regulation and enzymatic functions of this plant gene superfamily. Expand
Plant Glutathione Transferases in Abiotic Stress Response and Herbicide Resistance
This chapter discusses the involvement of plant GSTs in abiotic stress response with focus on metabolism-based herbicide resistance and attempts to give an overview of their catalytic roles and in planta function. Expand
Catalytic and structural diversity of the fluazifop-inducible glutathione transferases from Phaseolus vulgaris
The results showed that the fluazifop-inducible glutathione transferases from P. vulgaris catalyze a broad range of reactions and exhibit quite varied substrate specificity. Expand
Cloning and Characterization of a Biotic-Stress-Inducible Glutathione Transferase from Phaseolus vulgaris
The catalytic diversification of a GST isoenzyme from Phaseolus vulgaris which is induced under biotic stress treatment is systematically examined and shows high antioxidant catalytic function and acts as hydroperoxidase, thioltransferase, and dehydroascorbate reductase. Expand
Characterization and functional analysis of a recombinant tau class glutathione transferase GmGSTU2-2 from Glycine max.
The results established that GmGSTU2-2 participates in a broad network of catalytic and regulatory functions involved in the plant stress response, and suggested a highly specific and selective induction pattern to osmotic stresses. Expand
Effect of under‐ and overfeeding on sheep and goat milk and plasma enzymes activities related to oxidation
The results showed that the underfed sheep in comparison with the control had significantly lower glutathione reductase (GR), superoxide dismutase (SOD), catalase and glutATHione peroxidase (GPX) activities and total antioxidant capacity (measured with Ferric Reducing Ability of Plasma [FRAP] assay) in their blood plasma. Expand
The impact of the dietary supplementation level with schizochytrium sp., on the oxidative capacity of both goats' organism and milk.
Abstract The enrichment of milk with polyunsaturated fatty acids (PUFA) can be achieved with the dietary supplementation with microalgae. However, due to the fact that PUFA are prone to oxidation,Expand
Structure, Evolution and Functional Roles of Plant Glutathione Transferases
This chapter focuses on plant GSTs and attempts to give an overview of their evolution, catalytic function and structural biology. Expand