• Publications
  • Influence
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
TLDR
This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
The new frontier of genome engineering with CRISPR-Cas9
TLDR
The power of the CRISPR-Cas9 technology to systematically analyze gene functions in mammalian cells, study genomic rearrangements and the progression of cancers or other diseases, and potentially correct genetic mutations responsible for inherited disorders is illustrated.
Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation
TLDR
To compare the architectures and domain organization of diverse Cas9 proteins, the atomic structures of Cas9 from Streptococcus pyogenes and Actinomyces naeslundii and AnaCas9 were determined by x-ray crystallography and three-dimensional reconstructions of apo-SpyCas9, SpyCas9:RNA, and SpyCas 9:RNA:DNA were obtained by negative-stain single-particle electron microscopy.
The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA
TLDR
It is shown that type V-A Cpf1 from Francisella novicida is a dual-nuclease that is specific to crRNA biogenesis and target DNA interference and constitutes the most minimalistic of the CRISPR–Cas systems so far described.
Antibiotic Resistance in Listeriaspp
TLDR
This review will focus on the recent emergence of antibiotic resistance in Listeria spp.
Emergence of vancomycin tolerance in Streptococcus pneumoniae
TLDR
It is concluded that broad antibiotic tolerance of S.pneumoniae has emerged in the community by a molecular mechanism that eliminates sensitivity to the current cornerstone of therapy, vancomycin.
McsB Is a Protein Arginine Kinase That Phosphorylates and Inhibits the Heat-Shock Regulator CtsR
TLDR
Structural and biochemical studies show that McsB inhibits DNA binding of CtsR by specifically phosphorylating arginine residues in its DNA binding domain, thereby impairing its function as a repressor of stress response genes.
Incidence of antibiotic resistance in Listeria species.
TLDR
Data suggest transfer from the latter to Listeria species, as well as the tet(M), tet(S), and aad6 genes are common in enterococci and streptococci, suggest uniform susceptibility to tetracycline, minocyCline, trimethoprim, and Streptomycin cannot be assumed any longer for Listerian species.
Adopt a moratorium on heritable genome editing
Eric Lander, Françoise Baylis, Feng Zhang, Emmanuelle Charpentier, Paul Berg and specialists from seven countries call for an international governance framework.Eric Lander, Françoise Baylis, Feng
Identification of a Streptococcus pneumoniae Gene Locus Encoding Proteins of an ABC Phosphate Transporter and a Two-Component Regulatory System
TLDR
A more complex role of the Pst system in pneumococcal physiology than in that of E. coli is suggested, as mutagenesis of the ATP-binding cassette gene, pstB, resulted in decreased uptake of phosphate, and withdrawal of phosphate led to transformation deficiency in the parent strain R6x but not to penicillin tolerance, suggesting that reduced bacterial death was independent of phosphate.
...
...