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Isolation and biological activity of the proteases released by sea urchin eggs following fertilization.
Abstract The protease activity released from sea urchin egg cortical granules into the surrounding seawater at fertilization is involved in vitelline layer elevation and the block to polyspermy. TheExpand
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A method for hormonal induction of sperm release in anurans (eight species) and in vitro fertilization in Lepidobatrachus species
Injections of synthetic human gonadotropin releasing hormone (GnRH) into the dorsal pelvic area were used in an attempt to stimulate sperm release in isolated males of eight anuran species includingExpand
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Is calcium ionophore a universal activator for unfertilised eggs?
WE have previously exposed sea urchin eggs to micromolar amounts of the divalent transporting ionophore A23187, and observed every parameter of normal fertilisation. The cortical reaction withExpand
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Structure and Macromolecular Composition of the Egg and Embryo Jelly Coats of the Anuran Lepidobatrachus laevis
Eggs and cleavage‐stage embryos of the frog Lepidobatrachus laevis are encased by 3 μm thick vitelline/fertilization envelope and two jelly layers, termed J1 (innermost) and J2 (outermost). Based onExpand
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Resolution and characterization of a major protein of the sea urchin hyaline layer.
A major protein component of the gel-like, embryonic hyaline layer of Strongylocentrotus purpuratus has been purified and characterized. The protein retains the ability to form an insoluble gel inExpand
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Sea urchin embryo fertilization envelope: immunological evidence that soluble envelope proteins are derived from cortical granule secretions.
Abstract Although structural studies support the hypothesis that the sea urchin embryo fertilization envelope is derived from the preexisting vitelline envelope template and structural proteinsExpand
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Partial purification and characterization of sperm receptor hydrolase, a cortical granule proteoesterase, from eggs of the sea urchin Strongylocentrotus purpuratus
Sperm receptor hydrolase, one of two classes of cortical granule proteoesterases (E.C.3.4.4.4) was purified approximately 30-fold with 80% yield from Strongylocentrotus purpuratus cortical granuleExpand
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Structure, assembly and function of the surface envelope (fertilization envelope) from eggs of the sea urchin, Strongylocentrotus purpuratus.
The sea urchin fertilization envelope (FE) is formed following initial sperm-egg interaction from the egg surface vitelline envelope (VE) and the paracrystalline protein fraction (PCF), derived fromExpand
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[28] Cortical granule proteases from sea urchin eggs
Publisher Summary Sea urchin eggs contain protease activity in the cortical granules, a set of secretory organelles adjacent to the plasma membrane. After the fusion of cortical granule and plasmaExpand
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A calcium-insoluble 6.4 S protein derived from sea urchin cortical granule exudate.
A major protein component of the sea urchin, Strongylocentrotus purpuratus, cortical granule exudate has been purified and characterized. In the absence of divalent cations, the native, solubleExpand
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