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Reversible histone modifications and the chromosome cell cycle.
  • E. Bradbury
  • Biology, Medicine
  • BioEssays : news and reviews in molecular…
  • 1992
The subunits of histone H1 kinase have now been shown to be cyclins and the p34CDC2 kinase product of the cell cycle control gene CDC2, probable that all of the processes that control chromosome structure:function relationships are also involved in the control of thecell cycle. Expand
High-resolution proton-magnetic-resonance studies of chromatin core particles.
The binding of histones in chromatin core particles and in core particles depleted of histones H2A and H2B has been studied by high-resolution proton nuclear magnetic resonance (NMR) at 270 MHZ. AtExpand
Higher-order structures of chromatin in solution.
A family of supercoils of nucleosomes which contract with increasing ionic strength is suggested which is based on the structure of the core particle and various arrangement of core particles in the nucleofilament. Expand
The subunit structure of the eukaryotic chromosome
New structural data have been obtained from neutron scattering studies of chromatin. The concentration-dependent meridional peak at 10–11 nm comes from the interparticle spacing of a subunitExpand
The conformation of histone H5. Isolation and characterisation of the globular segment.
It is concluded that the resistant peptide, GH5, represents a globular folded region of the molecule whilst the rapidly digested parts are disordered and remains all the alpha-helical structure of intact H5 and appears to also maintain all the tertiary structure. Expand
Small angle neutron scattering studies of chromatin subunits in solution
A spherically averaged structure with most of the histones closely packed into a core of radius 3.2 nm surrounded by a loosely packed DNA-rich shell of 2.0 nm thickness is proposed resulting in a particle of 5. Expand
Acetylation of histone H4 and its role in chromatin structure and function
Testing H4 acetate content as a function of the cell cycle using the naturally synchronous cell cycle in Physarum polycephalum shows two clear correlations: tetra-acetylated H4 correlates with transcription and highly acetylation H4 is inversely correlated with HI phosphorylation and initiation of chromosome condensation in prophase. Expand
Studies on the role and mode of operation of the very-lysine-rich histone H1 in eukaryote chromatin. The three structural regions of the histone H1 molecule.
Proton nuclear magnetic resonance (NMR) and ultracentrifugation studies show that the H1 histone molecule consists of three distinct structural domains under structuring conditions: a random coil 'nose' consisting of 35 to 40 residues from the N-terminal end; a globular 'head' involving the next approximately 80 residues; and a random-coil 'tail' of the remainder of the molecule. Expand
Studies on the role and mode of operation of the very-lysine-rich histone H1 in eukaryote chromatin. The isolation of the globular and non-globular regions of the histone H1 molecule.
It seems that the structure of the H1 histone in solution under physiological conditions consists of a globular head with a highly basic random coil tail. Expand
Identification of ubiquitinated histones 2A and 2B in Physarum polycephalum. Disappearance of these proteins at metaphase and reappearance at anaphase.
Detailed studies of mitosis in Physarum polycephalum macroplasmodia suggest that cleavage of ubiquitin from the uH 2As and uH2B is a very late, possibly final event in chromosome condensation to metaphase chromosomes and that ubiquitination is an early event in their decondensation. Expand