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- Publications
- Influence
Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
- E. Betzig, G. Patterson, +6 authors H. Hess
- Biology, Medicine
- Science
- 15 September 2006
We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized… Expand
Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
- Bi-Chang Chen, Wesley R. Legant, +24 authors E. Betzig
- Materials Science, Medicine
- Science
- 24 October 2014
Introduction In vivo imaging provides a window into the spatially complex, rapidly evolving physiology of the cell that structural imaging alone cannot. However, observing this physiology directly… Expand
High-density mapping of single-molecule trajectories with photoactivated localization microscopy
- S. Manley, J. Gillette, +4 authors J. Lippincott-Schwartz
- Physics, Medicine
- Nature Methods
- 1 February 2008
We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions… Expand
Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics
- H. Shroff, C. Galbraith, J. A. Galbraith, E. Betzig
- Materials Science, Medicine
- Nature Methods
- 1 May 2008
We demonstrate live-cell super-resolution imaging using photoactivated localization microscopy (PALM). The use of photon-tolerant cell lines in combination with the high resolution and molecular… Expand
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
- T. Planchon, L. Gao, +4 authors E. Betzig
- Materials Science, Medicine
- Nature Methods
- 1 May 2011
A key challenge when imaging living cells is how to noninvasively extract the most spatiotemporal information possible. Unlike popular wide-field and confocal methods, plane-illumination microscopy… Expand
Single-Molecule Dynamics of Enhanceosome Assembly in Embryonic Stem Cells
- J. Chen, Zhengjian Zhang, +9 authors Z. Liu
- Biology, Medicine
- Cell
- 13 March 2014
Enhancer-binding pluripotency regulators (Sox2 and Oct4) play a seminal role in embryonic stem (ES) cell-specific gene regulation. Here, we combine in vivo and in vitro single-molecule imaging,… Expand
Single-Molecule Discrimination of Discrete Perisynaptic and Distributed Sites of Actin Filament Assembly within Dendritic Spines
- N. Frost, H. Shroff, Huihui Kong, E. Betzig, T. Blanpied
- Biology, Medicine
- Neuron
- 15 July 2010
Within dendritic spines, actin is presumed to anchor receptors in the postsynaptic density and play numerous roles regulating synaptic transmission. However, the submicron dimensions of spines have… Expand
Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans
- Jennifer T Wang, J. Smith, +7 authors G. Seydoux
- Biology, Medicine
- eLife
- 23 December 2014
RNA granules have been likened to liquid droplets whose dynamics depend on the controlled dissolution and condensation of internal components. The molecules and reactions that drive these dynamics in… Expand
Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes
- H. Shroff, C. Galbraith, +5 authors E. Betzig
- Biology, Medicine
- Proceedings of the National Academy of Sciences
- 18 December 2007
Accurate determination of the relative positions of proteins within localized regions of the cell is essential for understanding their biological function. Although fluorescent fusion proteins are… Expand
Self-Organization of the Escherichia coli Chemotaxis Network Imaged with Super-Resolution Light Microscopy
- D. Greenfield, A. McEvoy, +4 authors J. Liphardt
- Biology, Medicine
- PLoS biology
- 1 June 2009
Photoactivated localization microscopy analysis of chemotaxis receptors in bacteria suggests that the non-random organization of these proteins results from random self-assembly of clusters without… Expand