E. Betzig

Publications191
h-index70
Citations30,624
Highly Influential Citations1,045
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Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
TLDR
This work introduced a method for optically imaging intracellular proteins at nanometer spatial resolution and used this method to image specific target proteins in thin sections of lysosomes and mitochondria and in fixed whole cells to image retroviral protein Gag at the plasma membrane.
Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
TLDR
A new microscope using ultrathin light sheets derived from two-dimensional optical lattices is developed, demonstrating the performance advantages of lattice light-sheet microscopy compared with previous techniques and highlighted phenomena that, when seen at increased spatiotemporal detail, may hint at previously unknown biological mechanisms.
High-density mapping of single-molecule trajectories with photoactivated localization microscopy
TLDR
This work created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables.
Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics
TLDR
By allowing observation of a wide variety of nanoscale dynamics, live-cell PALM provides insights into molecular assembly during the initiation, maturation and dissolution of cellular processes.
Single-Molecule Dynamics of Enhanceosome Assembly in Embryonic Stem Cells
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
TLDR
Scanned Bessel beams are used in conjunction with structured illumination and/or two-photon excitation to create thinner light sheets better suited to three-dimensional (3D) subcellular imaging.
Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans
TLDR
Evidence is presented that a group of intrinsically disordered, serine-rich proteins regulate the dynamics of P granules in C. elegans embryos, and it is concluded that P granule assembly in embryos is regulated by phosphorylation.
Actin Depletion Initiates Events Leading to Granule Secretion at the Immunological Synapse
Self-Organization of the Escherichia coli Chemotaxis Network Imaged with Super-Resolution Light Microscopy
Photoactivated localization microscopy analysis of chemotaxis receptors in bacteria suggests that the non-random organization of these proteins results from random self-assembly of clusters without…
Single-Molecule Discrimination of Discrete Perisynaptic and Distributed Sites of Actin Filament Assembly within Dendritic Spines
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