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The RasGAP-associated endoribonuclease G3BP assembles stress granules
Stress granules (SGs) are formed in the cytoplasm in response to various toxic agents, and are believed to play a critical role in the regulation of mRNA metabolism during stress. In SGs, mRNAs areExpand
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Inhibition of Translational Initiation by Let-7 MicroRNA in Human Cells
MicroRNAs (miRNAs) are ∼21-nucleotide-long RNA molecules regulating gene expression in multicellular eukaryotes. In metazoa, miRNAs act by imperfectly base-pairing with the 3′ untranslated region ofExpand
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Localization of ASH1 mRNA particles in living yeast.
ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of HO expression, important for mating type switching. To visualize real time localization of the mRNA in livingExpand
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Human telomerase RNA and box H/ACA scaRNAs share a common Cajal body–specific localization signal
Telomerase is a ribonucleoprotein reverse transcriptase that uses its RNA component as a template for synthesis of telomeric DNA repeats at the ends of linear eukaryotic chromosomes. Here,Expand
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From Silencing to Gene Expression Real-Time Analysis in Single Cells
We have developed an inducible system to visualize gene expression at the levels of DNA, RNA and protein in living cells. The system is composed of a 200 copy transgene array integrated into aExpand
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Single mRNA Molecules Demonstrate Probabilistic Movement in Living Mammalian Cells
Cytoplasmic mRNA movements ultimately determine the spatial distribution of protein synthesis. Although some mRNAs are compartmentalized in cytoplasmic regions, most mRNAs, such as housekeeping mRNAsExpand
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Modification of Sm small nuclear RNAs occurs in the nucleoplasmic Cajal body following import from the cytoplasm
Biogenesis of functional spliceosomal small nuclear RNAs (snRNAs) includes the post‐transcriptional covalent modification of numerous internal nucleotides. We have recently demonstrated thatExpand
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Hypermethylation of the cap structure of both yeast snRNAs and snoRNAs requires a conserved methyltransferase that is localized to the nucleolus.
The m(7)G caps of most spliceosomal snRNAs and certain snoRNAs are converted posttranscriptionally to 2,2,7-trimethylguanosine (m(3)G) cap structures. Here, we show that yeast Tgs1p, anExpand
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Cajal body‐specific small nuclear RNAs: a novel class of 2′‐O‐methylation and pseudouridylation guide RNAs
Cajal (coiled) bodies are conserved subnuclear organelles that are present in the nucleoplasm of both animal and plant cells. Although Cajal bodies were first described nearly 100 years ago, theirExpand
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The human cap-binding complex is functionally connected to the nuclear RNA exosome
Nuclear processing and quality control of eukaryotic RNA is mediated by the RNA exosome, which is regulated by accessory factors. However, the mechanism of exosome recruitment to itsExpand
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