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The Arabidopsis HKT1 gene homolog mediates inward Na(+) currents in xenopus laevis oocytes and Na(+) uptake in Saccharomyces cerevisiae.
The isolation of a cDNA homologous to HKT1 from Arabidopsis and the characterization of its mode of ion transport in heterologous systems show that AtHKT1 can mediate Na(+) and, to a small degree, K(+) transport inheterologous expression systems.
Glycine residues in potassium channel-like selectivity filters determine potassium selectivity in four-loop-per-subunit HKT transporters from plants
Experimental evidence is provided that the glycine residues in selectivity filters of HKT proteins are structurally related to those of K+ channels.
Transient accumulation of potassium glutamate and its replacement by trehalose during adaptation of growing cells of Escherichia coli K-12 to elevated sodium chloride concentrations
Results indicate that for osmoadaptation of growing cells of E. coli the uptake of proline has priority over the synthesis of trehalose, which in its turn is preferred above K+ and glutamate as osmoprotectants.
Requirement of a large K+-uptake capacity and of extracytoplasmic protease activity for protamine resistance of Escherichia coli
Data on the effect of a second addition of protamine suggest that protamine degradation activity is inducible, which is interpreted to mean that reaccumulation of K+ by protamine-treated cells triggers recovery of the cells, thereby allowing induction of extracytoplasmic proteases.
The Escherichia coli MotAB proton channel unplugged.
Alkali Cation Transport Systems in Prokaryotes
- E. Bakker
- 30 November 1992
Na+ Transport Systems: Na+ Transport systems in Prokaryotes, and Regulation of Gene Expression during Osmoregulation: The Role of Potassium Glutamate as a Secondary Signal For Osmotic Stress.
Interconversion of components of the bacterial proton motive force by electrogenic potassium transport
The data are interpreted to mean that the inward movement of K+ ions via each of these bacterial transport systems is electrogenic, which leads to a depolarization of the membrane, which in its turn allows the cell to pump more protons into the medium.
Evolutionary relationship between K(+) channels and symporters.
Purification, properties and structural aspects of a thermoacidophilic alpha-amylase from Alicyclobacillus acidocaldarius atcc 27009. Insight into acidostability of proteins.
- B. Schwermann, K. Pfau, B. Liliensiek, M. Schleyer, T. Fischer, E. Bakker
- Biology, ChemistryEuropean journal of biochemistry
- 1 December 1994
It is proposed that these proteins have adapted to the acidic environment by reducing the density of both positive and negative charges at their surface, that this effect circumvents electrostatic repulsion of charged groups at low pH, and thereby contributes to the acidostability of these proteins.
Identification of OmpT as the Protease That Hydrolyzes the Antimicrobial Peptide Protamine before It Enters Growing Cells ofEscherichia coli
- S. Stumpe, R. Schmid, D. Stephens, G. Georgiou, E. Bakker
- BiologyJournal of bacteriology
- 1 August 1998
Data indicate that OmpT is the protease that degrades protamine and that it exerts this function at the external face of the outer membrane.