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The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil
The cell content of 12 bacterial phospholipid fatty acids (PLFA) was determined in bacteria extracted from soil by homogenization/centrifugation and the soil content of the PLFA 18:2ω6 was correlated with the ergosterol content, which supports the use of this PLFA as an indicator of fungal biomass. Expand
Soil bacterial and fungal communities across a pH gradient in an arable soil
Soils collected across a long-term liming experiment were used to investigate the direct influence of pH on the abundance and composition of the two major soil microbial taxa, fungi and bacteria, and both the relative abundance and diversity of bacteria were positively related to pH. Expand
Phospholipid Fatty Acid Composition, Biomass, and Activity of Microbial Communities from Two Soil Types Experimentally Exposed to Different Heavy Metals
Effects on the PLFA patterns were found at levels of metal contamination similar to or lower than those at which effects on ATP content, soil respiration, or total amount of PLFAs had occurred. Expand
Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis
Analysis of phospholipid fatty acid composition of soils from two areas in the south of Sweden indicated that the increased pH caused a shift in the bacterial community to more Gram-negative and fewer Gram-positive bacteria, while the amount of fungi was unaffected. Expand
Estimation of the biomass and seasonal growth of external mycelium of ectomycorrhizal fungi in the field.
The biomass of EM mycelium in the soil was in the same range as the biomass of fine roots and peaks of mycelial growth coincided with periods of maximum growth of fine-roots. Expand
Contrasting Soil pH Effects on Fungal and Bacterial Growth Suggest Functional Redundancy in Carbon Mineralization
ABSTRACT The influence of pH on the relative importance of the two principal decomposer groups in soil, fungi and bacteria, was investigated along a continuous soil pH gradient at Hoosfield acidExpand
Use and misuse of PLFA measurements in soils
The determination of the phospholipid fatty acid (PLFA) pattern of soil organisms has become one of the most commonly used methods to study microbial community structure. Here we recapitulate theExpand
Microbial biomass measured as total lipid phosphate in soils of different organic content
The use of total lipid phosphate as a measure of biomass was evaluated in soils with different organic matter content. Lipids were extracted with a one-phase mixture of chloroform, methanol, and aExpand
Comparison of soil fungal/bacterial ratios in a pH gradient using physiological and PLFA-based techniques
The total microbial biomass and the fungal/bacterial ratio estimated using substrate-induced respiration (SIR) in combination with the selective inhibition technique and using the phospholipid fatty acid (PLFA) technique in a pH gradient indicating that the microbial community composition in these beech/beech-oak forest soils was to a large extent determined by soil pH. Expand
Effects of heavy metals in soil on microbial processes and populations (a review)
The effects of Cd, Cu, Zn, and Pb on soil microorganisms and microbially mediated soil processes are reviewed. The emphasis is placed on temperate forest soils. The sensitivity of differentExpand