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Recent advances in the bioremediation of persistent organic pollutants via biomolecular engineering
With recent advances in biomolecular engineering, the bioremediation of persistent organic pollutants (POPs) using genetically modified microorganisms has become a rapidly growing area of researchExpand
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A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae.
Despite recent advances in genome editing capabilities for the model organism Saccharomyces cerevisiae, the chromosomal integration of large biochemical pathways for stable industrial productionExpand
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CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters
Here we report an efficient CRISPR-Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs ofExpand
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Engineering microbial hosts for production of bacterial natural products.
Covering up to end 2015Microbial fermentation provides an attractive alternative to chemical synthesis for the production of structurally complex natural products. In most cases, however, productionExpand
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Using natural products for drug discovery: the impact of the genomics era
ABSTRACT Introduction: Evolutionarily selected over billions of years for their interactions with biomolecules, natural products have been and continue to be a major source of pharmaceuticals. In theExpand
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Engineered Pentafunctional Minicellulosome for Simultaneous Saccharification and Ethanol Fermentation in Saccharomyces cerevisiae
ABSTRACT Several yeast strains have been engineered to express different cellulases to achieve simultaneous saccharification and fermentation of lignocellulosic materials. However, successes in theseExpand
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Genome-wide identification of natural RNA aptamers in prokaryotes and eukaryotes
RNAs are well-suited to act as cellular sensors that detect and respond to metabolite changes in the environment, due to their ability to fold into complex structures. Here, we introduce aExpand
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Recent advances in combinatorial biosynthesis for drug discovery
Because of extraordinary structural diversity and broad biological activities, natural products have played a significant role in drug discovery. These therapeutically important secondary metabolitesExpand
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Biomolecular Engineering
Biomolecular engineering is an emerging field of academic research and industrial practice having the goal of engineering value-added biomolecules and biomolecular processes for applications inExpand
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Auroramycin: A Potent Antibiotic from Streptomyces roseosporus by CRISPR‐Cas9 Activation
Silent biosynthetic gene clusters represent a potentially rich source of new bioactive compounds. We report the discovery, characterization, and biosynthesis of a novel doubly glycosylatedExpand
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