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Quantitative Analysis of Bacterial Toxin Affinity and Specificity for Glycolipid Receptors by Surface Plasmon Resonance*
The primary virulence factors of many pathogenic bacteria are secreted protein toxins which bind to glycolipid receptors on host cell surfaces. The binding specificities of three such toxins forExpand
Production of exopolysaccharide by Burkholderia cenocepacia results in altered cell-surface interactions and altered bacterial clearance in mice.
This mucoid colonial appearance of Burkholderia cenocepacia could enhance the persistence and virulence of this important CF-related pathogen. Expand
Structural characteristics of the antigenic capsular polysaccharides and lipopolysaccharides involved in the serological classification of Actinobacillus (Haemophilus) pleuropneumoniae strains
Abstract The detailed structures of the specific capsular polysaccharides and cellular lipopolysaccharides of the 12 known serotypes of Actinobacillus (Haemophilus) pleuropneumoniae are presented andExpand
The HeLa cell receptor for enterovirus 70 is decay-accelerating factor (CD55)
Data indicate that the HeLa cell receptor for EV70 is DAF, a Decay-accelerating factor-anchored membrane protein that is involved in the regulation of complement and has also been shown to function as a receptor for several enteroviruses. Expand
Structural elucidation of the lipopolysaccharide core regions of the wild-type strain PAO1 and O-chain-deficient mutant strains AK1401 and AK1012 from Pseudomonas aeruginosa serotype O5.
Deoxycholate/PAGE of the LPS from the rough mutant AK1401 indicated two bands near the dye front with mobilities similar to those of the parent strain, indicating that both LPS contain a complete core and a species comprising a core and one repeating unit. Expand
Functional genomics of Helicobacter pylori: identification of a β‐1,4 galactosyltransferase and generation of mutants with altered lipopolysaccharide
Mutational analysis in H. pylori strains demonstrated that this enzyme plays a key role in the biosynthesis of the type 2 N‐acetyl‐lactosamine (LacNAc) polysaccharide O‐chain backbone, by catalysing the addition of Gal to GlcNAc. Expand
Structure of the capsular polysaccharide of Haemophilus pleuropneumoniae serotype 5.
It was concluded that the capsular polysaccharide of Haemophilus (Actinobacillus) pleuropneumoniae serotype 5 is a high-molecular-mass unbranched polymer having the structure: [6)-alpha-D-GlcNAcp-(1-5)-beta-dOclAp-(2]n. Expand
Characterization of lipopolysaccharide-deficient mutants of Pseudomonas aeruginosa derived from serotypes O3, O5, and O6
A first look at the core oligosaccharide region of serotype O6 LPS obtained with the O6-derived mutants generated in this study is obtained. Expand
Structural studies of the core region of Aeromonas salmonicida subsp. salmonicida lipopolysaccharide.
The core oligosaccharide structure of the in vivo derived rough phenotype of Aeromonas salmonicida subsp. salmonicida was investigated by a combination of compositional, methylation, CE-MS and one-Expand
Evidence for the extended helical nature of polysaccharide epitopes. The 2.8 A resolution structure and thermodynamics of ligand binding of an antigen binding fragment specific for
X-ray crystallographic and thermodynamic evidence are consistent with a binding site that accommodates at least eight sialic acid residues, and a model of antigen binding is presented that satisfies this thermodynamic data, as well as a previously reported requirement of conformational specificity of the oligosaccharide. Expand