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454 pyrosequencing analyses of bacterial and archaeal richness in 21 full-scale biogas digesters.
TLDR
The microbial community of 21 full-scale biogas reactors was examined using 454 pyrosequencing of 16S rRNA gene sequences and revealed that methane formation from acetate takes place mainly via syntrophic acetate oxidation in the CD reactors.
Geographical and temporal distribution of SARS-CoV-2 clades in the WHO European Region, January to June 2020
TLDR
The importance of real-time sequencing and data dissemination in a pandemic situation is highlighted, the nomenclatures are compared, and a foundation for future European genomic surveillance of SARS-CoV-2 is laid.
Universal Single-Probe RT-PCR Assay for Diagnosis of Dengue Virus Infections
TLDR
The validation of the RT-PCR assay presented here indicates that this technique can be a reliable diagnostic tool, and hence it is suggested that it be introduced as the method of choice during the first 5 days of dengue symptoms.
One-step real-time RT-PCR assays for serotyping dengue virus in clinical samples
TLDR
The design and validation of one-step real-time serotype-specific DENV RT-PCR assays are described, finding them to be specific for their respective serotype and did not cross-react with other flaviviruses or human mRNA.
A novel real-time PCR assay for specific detection of Brucella melitensis
TLDR
This new real-time PCR method shows a high specificity (100%) and a high sensitivity (1.25 GE/μl) and has been implemented in the laboratories of four governmental authorities across Sweden.
Monitoring of SARS‐CoV‐2 infection in mustelids
TLDR
American mink and ferret are highly susceptible to severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), but no information is available for other mustelid species, and all mink farms should be considered at risk of infection.
Non-pncA Gene-Mutated but Pyrazinamide-Resistant Mycobacterium tuberculosis: Why Is That?
TLDR
It is necessary to consider the occurrence of panD mutations in PZA-resistant isolates, as well as heteroresistance, for the development and evaluation of new molecular techniques to ensure high-quality DST performance.
A nationwide outbreak of listeriosis associated with cold-cuts, Sweden 2013-2014
TLDR
E Epidemiological and microbiological results indicated cold-cuts as a possible source of the listeriosis outbreak, and whole-genome sequencing showed that cold-cut and patient isolates differed by eight single nucleotide polymorphisms.
A note on normalization of biofluid 1D 1H-NMR data
TLDR
It is shown that HM is a robust method for normalizing 1H-NMR data and proposed it as an alternative to the traditional CS method and validated using mixtures of synthetic compounds that mimic a biological extract.
Proof of principle of a generalized fuzzy Hough transform approach to peak alignment of one-dimensional 1H NMR data
TLDR
A new algorithm is proposed, based on the Hough transform operating on an image representation of the NMR dataset that is capable of correctly aligning peaks when existing methods fail, and was compared with current state-of-the-art algorithms operating on a selected plasma dataset to demonstrate its potential.
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