E. Wibe

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  • E. Wibe
  • 1980
DURING the last 10 years the in vitro tumour model called "multicellular spher-oids" has proved to be an important link between tumours in vivo and standard in vitro cell cultures. Sutherland et al. (1970) wheni introducing multicellular spheroids for the first time, showed by means of histological sections a striking morphological resemblance between(More)
Cells from the established cell line NHIK 3025 were synchronized by repeated mitotic selections. Survival of the synchronized cells after treatment with haematoporphyrin and near-UV light was measured by testing the capacity of the cells to form macroscopic colonies. The sensitivity to photodynamic inactivation was small in early G1, late S and G2. The(More)
Effects of the mitotic inhibitor NY 3170 (1-propargyl-5-chloropyrimidin-2-one) on cell-cycle kinetics of NHIK 3025 cells were studied by means of time-lapse microcinematography, pulsed incorporation of [3H] thymidine, flow cytometry, and mitotic index. All the experiments were performed with cells synchronized by mitotic selection. Mitotic inhibition as(More)
Inactivating effects caused by vincristine alone or in combination with another mitotic inhibitor, 1-propargyl-5-chloropyrimidin-2-one, were studied as loss of colony-forming ability in exponentially growing or synchronized populations of the human cell line NHIK 3025. Treatment with 4 ng vincristine per ml(4.3 nM) in G2 led to irreversible mitotic arrest.(More)
Inactivation of NHIK 3025 cells ny the mitotic inhibitor NY 3170 (1-propargyl-5-chloropyrimidin-2-one) was measured as loss of colony-forming ability. NY 3170 at a concentration of 0.15 nM allowed no formation of colonies after 12 days of continuous exposure to the drug. Metaphase arrest after treatment with NY 3170 was reversible if the drug was removed(More)
By means of flow cytometric recording of DNA histograms and counting of cells in synchronized populations, we have found that misonidazole (MIS) in clinically relevant concentrations induces cell-kinetic changes in human cells (NHIK 3025) cultivated in vitro under aerobic conditions. The effect seems to be a general lengthening of the cell cycle, affecting(More)
By means of time-lapse microcinematography, meta-phase arrest was studied in synchronized NHIK 3025 cells in contact with 5-chloropyrimidin-2-one (NY 3000) or vin-cristine. A dose-dependent prolongation of mitosis was found for both substances, and the fraction of cells able to escape mitotic arrest declined gradually as the concen tration of NY 3000 or(More)
The effect of different steroids on the growth of a human cell line, was examined in NHIK 3025 cells, derived from a carcinoma of the uterine cervix. When this cell line was grown in Eagle's minimal essential medium (MEM) for 4 days, addition to the medium of estradiol-17 beta in concentrations ranging from 10(-9) to 10(-7) M did not promote significant(More)
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