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This work studies the extraction of intracellular components from microalgae Nannochloropsis sp. with application of different cell disruption techniques, including pulsed electric field (PEF) (20kV/cm, 1-4ms, 13.3-53.1kJ/kg), high voltage electrical discharge (HVED) (40kV/cm, 1-4ms, 13.3-53.1kJ/kg), ultrasonication (USN) (200W, 1-8min, 12-96kJ/kg), and(More)
A computer model was developed for the estimation of the kinetics of microbial inactivation by pulsed electric field (PEF). The model is based on the electroporation theory of individual membrane damage, where spherical cell geometry and distribution of cell sizes are assumed. The variation of microbial cell sizes was assumed to follow a statistical(More)
Electroporation is a method of treatment of plant tissue that due to its nonthermal nature enables preservation of the natural quality, colour and vitamin composition of food products. The range of processes where electroporation was shown to preserve quality, increase extract yield or optimize energy input into the process is overwhelming, though not(More)
Fossil resources-free sustainable development can be achieved through a transition to bioeconomy, an economy based on sustainable biomass-derived food, feed, chemicals, materials, and fuels. However, the transition to bioeconomy requires development of new energy-efficient technologies and processes to manipulate biomass feed stocks and their conversion(More)
The aim of this study was to optimise the electrically assisted extraction in order to obtain grape pomace extracts with high polyphenols content, which would be potentially interesting for applications as natural antioxidants. High voltage electrical discharges (HVED) were applied for intensification of the extraction. The effects of the energy input, the(More)
Pulsed electric field (PEF) treatment is an emerging technology that is arousing increasing interest in vinification processes for its ability to enhance polyphenol extraction performance. The aim of this study was to investigate the effects of PEF treatment on grape skin histocytological structures and on the organization of skin cell wall polysaccharides(More)
The objective of this study is to provide insight into the phenomena related with juice expression from electroporated tissue. We propose an analytical model and study consolidation behaviour of a block of tissue during pressing; before and after electroporation. By the dual-porosity approach, we treat com-pressibility and hydraulic permeability of(More)
The batch fermentation process, inoculated by pulsed electric field (PEF) treated wine yeasts (S. cerevisiae Actiflore F33), was studied. PEF treatment was applied to the aqueous yeast suspensions (0.12 % wt.) at the electric field strengths of E=100 and 6000 V/cm using the same pulse protocol (number of pulses of n=1000, pulse duration of ti=100 mks, and(More)
Pressurised electro-osmotic dewatering (PEOD) of two sewage sludges (activated and anaerobically digested) was studied under constant electric current (C.C.) and constant voltage (C.V.) with a laboratory chamber simulating closely an industrial filter. The influence of sludge characteristics, process parameters, and electrode/filter cloth position was(More)
This research employed a conductometric technique to estimate the inactivation kinetics of Escherichia coli cells in aqueous suspensions (1 wt.%) during simultaneous pulsed electric fields (PEF) and thermal treatments. The electric field strength was E=5 kV/cm, the effective PEF treatment time t(PEF) was within 0-0.2 s, the pulse duration t(i) was 10(-3) s,(More)