E V Zeĭmal'

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The response to electrophoretic application of acetylcholine and suberyldicholine was studied in two identified neurons (LPed-2 and LPed-3) isolated from Planorbarius corneus left pedal ganglion. Acetylcholine reversal potential was found to be significantly more negative with K(2)SO(4)-filled microelectrodes than with KCl-filled ones. Reversal potentials(More)
Acetylcholine (ACh) content of the central nervous system in the sipunculid P. japonicum was estimated by application of extracts from the nervous tissue to dental retractor of the sea urchin using ACh solutions with known concentration as a standard. It was shown that the nervous tissue contains 46 (from 41 to 51) microng of ACh (calculated as cation) per(More)
Furosemide (2.10(-4) to 1.10(-3) g/ml) was shown to prevent the increase of chloride conductance induced in isolated neurons of freshwater mollusc Planorbarius corneus by ionophoretic application of acetylcholine, suberyldicholine or gamma-aminobutyric acid. Furosemide caused no shift in the reversal potential of the chloride-dependent responses if the(More)
Two kinds of responses to cholinomimetics were found on the identified neuron (P-2) of Planorbarius corneus pedal ganglion using microelectrode technique. Nicotinomimetics caused depolization whereas some muscarinomimetics caused hyperpolarization of the neuron membrane. Acetylcholine usually depolarized the neuron membrane but after the blockade of(More)
In experiments on isolated and identified neurones of the pedal ganglion of the gastropod mollusc P. corneus, it was demonstrated that biphasic response to ACh may be obtained both to superfusion and electrophoretic application. Fast (depolarizing) phase may be imitated by a nicotinomimetic drug, suberyldicholine, and blocked by d-tubocurarine. These data(More)
An identified neuron (L Ped-2) of the Planorbarius corneus left pedal ganglion was isolated after the treatment of the ganglionic ring with pronase. The LPed-2 retains after the isolation the main features characteristic of this neuron in the ganglion. Nicotinomimetics (nicotine, suberyldicholine) produced depolarization whereas muscarinomimetics (F-2268,(More)
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