E. V. Malykh

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A procedure was developed for acylation of Bowman–Birk soybean proteinase inhibitor (BBI) by N-hydroxysuc-cinimide esters of oleic, linoleic, and α-linolenic acids in a dimethyl sulfoxide–dioxane–pyridine mixture. BBI derivatives containing two acylated amino groups were prepared with high yield. The use of the reversible modifier citraconic anhydride in(More)
The effect of modification of basic pancreatic trypsin inhibitor (BPTI) by derivatives of fatty acids (oleic, stearic) on the inhibition of bovine trypsin and human leukocyte elastase (HLE) was studied. Kinetic constants of interaction with trypsin and inhibition constants of both enzymes were determined. Hydrophobization of BPTI had virtually no effect on(More)
Polymeric particles formed by stearoyl-poly-N-vinylpyrrolidone (PVP-stear) of Mn = 2600 were obtained in aqueous solution, and their shape and size distribution were characterized. The size of the particles was shown to decrease with an increase in the ionic strength of the solution. Interaction of PVP-stear and its aggregates with model proteins(More)
A procedure was developed for the modification of basic pancreatic trypsin inhibitor (BPTI) by N-hydroxysuccinimide esters of oleic and stearic acids in a DMSO-DMF-dioxane-pyridine mixture with a temporary citraconyl protection of the amino group belonging to its active site. The BPTI derivatives containing from one to three acylated amino groups were(More)
The effect of acylation of Bowman–Birk soybean proteinase inhibitor (BBI) by derivatives of various unsaturated fatty acids on inhibition of trypsin, α-chymotrypsin, and human leukocyte elastase was investigated. Inhibition (K i) and kinetic (k ass, k diss) constants of interaction between proteases and acylated BBI derivatives were determined. For mono-,(More)
The lipidized derivatives of Bowman-Birk soybean protease inhibitor (BBI) containing one to three oleoyl groups were synthesized and characterized. The (ole)(1)- and (ole)(2)BBI were demonstrated to have 200- and 100-fold higher uptake into Caco-2 cell monolayers compared to native BBI. The acylated BBI had increased affinity to elastase-like proteases.(More)
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