E. Thangapandian

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The efficacy of a recombinant leptospiral lipoprotein LipL41 as an antigen for conducting enzyme-linked immunosorbent assay (ELISA) for diagnosis of bovine leptospirosis was evaluated. Using known positive and known negative cattle sera the recombinant antigen was found to be highly reactive in the concentration of 100 ng/well. Using a total of 321 field(More)
A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62(More)
Leptospirosis of animals and man is an important rereported in recent years are LipL32, LipL41 and OmpL1; they emerging infectious disease worldwide. In domestic animals are expressed in-vivo during infection. LipL32 and LipL41 it is an important cause of abortion, stillbirth, infertility, are important and surface-exposed leptospiral outer membrane(More)
Abbreviations: ELISA, enzyme-linked immunosorbent assay; EMJH, Ellinghausen–McCullough–Johnson– Harris; IPTG, isopropyl thio-β-D-galactoside; LB, Luria–Bertani; LPS, lipopolysaccharide; MAT, microscopic agglutination test; OMP, outer membrane protein; PCR, polymerase chain reaction; SDS-PAGE, sodium dodecyl sulphate polyacrylamide gel electrophoresis;(More)
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