E Schickel

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We report on an ELISA procedure for the quantitative analysis of total human leukocyte (PMN)-elastase, i.e., the simultaneous determination of (i) free (non-complexed) PMN-elastase and of (ii) PMN-elastase that is complexed to alpha 1 proteinase inhibitor. Simultaneous detection of both forms of PMN-elastase was achieved using a monoclonal antibody that(More)
A human keratinocyte cell line (HaCaT) was analysed for plasminogen activator activity. By immuno-capture and immuno-inhibition studies we obtained evidence that HaCaT cells synthesize and secrete urokinase-type plasminogen activator (uPA) and tissue-type PA (tPA). Zymographic analysis by using fibrin agar indicator gels indicated that part of the PA(More)
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