E. S. Bishop

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PicoGreen is a fluorescent probe that binds dsDNA and forms a highly luminescent complex when compared to the free dye in solution. This unique probe is widely used in DNA quantitation assays but has limited application in biophysical analysis of DNA and DNA-protein systems due to limited knowledge pertaining to its physical properties and characteristics(More)
PicoGreen (PG) is a fluorescent probe for both double-stranded DNA (dsDNA) detection and quantification based on its ability to form a luminescent complex with dsDNA as compared with the free dye in solution. To expand the sensitivity of PG detection, we have studied the spectral properties of PG, both free and in complex with DNA in solution, when the(More)
In this paper we provide both a theoretical and experimental analysis of the sensitivity of a DNA quantitation assay using a fluorescent chromophore which non-covalently binds dsDNA. It is well-known that the range of DNA concentrations available for fluorescence quantitation depends on the concentration of the chromophore, its affinity for nucleic acids,(More)
In this study, we have investigated the fluorescence properties of SYBR Green I (SG) dye and its interaction with double-stranded DNA (dsDNA). SG/dsDNA complexes were studied using various spectroscopic techniques, including fluorescence resonance energy transfer and time-resolved fluorescence techniques. It is shown that SG quenching in the free state has(More)
At present no method for glycohemoglobin (%HbA1c) is automated on a main-line analyzer to allow joint measurement with other indicators of diabetic control such as glucose and cholesterol. We describe an adaptation of a latex-enhanced competitive immunoassay for quantifying %HbA1c to the Dade International Dimension analyzer. After a manual hemolysis step,(More)
enrolled i n the undergraduate Community Planning Laboratory conducted the study during two consecutive academic quarters, Fall and Winter of the 2001-2002 academic year. To develop this project, the students worked in collaboration with, and with financial assistance from the giving us the opportunity to be involved with this project. We extend a special(More)
OVERVIEW One of the central purposes of the Adoption and Safe Families Act of 1997 (ASFA) is to facilitate timely adoptions for children in foster care who cannot be reunited with their birth parents by shortening the timeframe for terminating birth parents' rights to their children. This process is formally known as termination of parental rights or TPR. 1(More)
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