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A qualitative screening technique was developed for the detection of 2-amino-5-chloropyridine, a newly identified decomposition product of zopiclone and metabolites after alkaline hydrolysis of urine samples. The method was elaborated using a standard operation procedure (Merck Tox Screening System), combining a solid-phase extraction with reversed-phase(More)
A sensitive and specific radioimmunoassay has been developed, allowing the stereospecific detection of nanogram amounts of (+)- and (-)-enantiomers of zopiclone and its major metabolites in urine, without prior extraction or purification. Antisera were obtained from two series of four rabbits, immunized with optically pure (+)- and(More)
A fatality, attributed to a suicidal ingestion of zopiclone and subsequent death by drowning, is described. A radioimmunoassay was performed to prescreen for the presence of the analyte. HPLC analysis with fluorescence detection demonstrated a testicular tissue concentration of 2.2 micrograms/g zopiclone. Further toxicological analysis revealed also the(More)
A polarization fluoroimmunoassay was developed for the detected of N-desmethylzopiclone in urine and the reagents were adapted for use on the Vitalab Eclair analyser. Therefore, N-fluoresceinthiocarbamyldesmethylzopiclone was synthesized as a fluorescent tracer and used in combination with an existing pool of antibodies, raised against the hemisuccinyl(More)
The development of a specific and sensitive radioimmunoassay for the detection of N-desmethylzopiclone in urine samples is described. The assay will be used to pre-screen for N-desmethylzopiclone as an indication of zopiclone intake in a forensic and emergency context. N-Hemisuccinyldesmethylzopiclone has been prepared as a hapten. The immunization of(More)
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