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Three groups of eggs, intact, cumulus-free and zona-free, were inseminated in vitro with spermatozoa from C57BL/Kw male mice and incubated for 1-2 h. In fixed, toluidine blue-stained preparations, spermatozoa surrounding the eggs or attached to them remained colourless, while sperm heads that had penetrated the vitellus were deeply stained. Those in the(More)
8053 Background: We assessed the presence of melanoma cells in LY and BL in melanoma pts after LND with MM RT-PCR assay for the evaluation of disease outcomes. METHODS Between 05/2002 and 06/2005 we collected 24-hr LY in 207 stage III melanoma patients after radical LND (91 - completion LND after positive sentinel node biopsy - CLND and 116 - therapeutic(More)