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We have previously reported that astroglial cell lines derived from spontaneously immortalized mouse cerebellar cultures as well as primary astrocyte cultures express the mRNA of the alpha isoform of smooth muscle actin. In this report, we have used an antiserum specific for the alpha smooth muscle actin protein to investigate the presence and the pattern(More)
Astrocytes belong to the glial cell population and represent a major subclass of the CNS. Although different subtypes of astrocytes have been described according to their morphological characteristics, biochemical markers of each subtype of astrocytes are not yet available. We have thus undertaken to compare gene expression pattern of different astroglial(More)
The CNS is composed of neurons and glial cells (i.e., astrocytes, oligodendrocytes, and microglia). The brain communicates with the blood circulation through choroid plexus and meninges as well as through the blood-brain barrier. To identify transcripts specifically expressed in a distinct brain cell type, we have previously constructed a subtracted cDNA(More)
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