E L Miettinen

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Summary: Pregnant dogs were starved for 72 hr while controls were fasted overnight. Maternal starvation significantly reduced fetal birth weight (269 ± 7.2 versus 294 ± 4.4 g). Total caloric deprivation had no effect on maternal or fetal blood glucose concentrations at the time of delivery; however, fasting neonatal blood glucose levels were depressed(More)
The metabolic effects of previously induced alloxan diabetes were investigated among fasted newborn dogs during the first day of life. Maternal insulin-dependent diabetes resulted in enhanced maternal and fetal blood glucose and ketone bodies, while postnatal levels of these substrates were not altered. Plasma insulin levels were augmented in the fetus and(More)
Newborn pups were assigned to a fasting group or to a group receiving intravenous glucose alimentation. Glucose turnover was determined during steady state equilibration of simultaneously infused [6-3H] glucose. Thereafter, pups from each group received 0.625 g/Kg of either oral [U-14C] galactose or [U-14C] glucose. In fasted or intravenously alimented pups(More)
Glucose given to the newborn human may result in hyperglycemia, suggesting that its utilization is impaired at this developmental stage. Galactose is thought to be a more appropriate carbohydrate source for the newborn. The enzymes involved in hexose phosphorylation may, in part, be responsible for these observations. A key regulatory enzyme of hepatic(More)
Summary: The effects of maternal canine starvation (MCS) for 5 days on hepatic intermediates in fetus and fasted neonates were studied at 0, 3, 6, 9, and 24 h of age. Maternal starvation reduced the birth weights of the pups by 23% (232 ± 6 versus 300 ± 10 g). In the MCS fetuses, hepatic glycogen concentration was significantly decreased (416 versus 526(More)
Summary: The effect of maternal starvation for 5 days on circulating fuels, on glucose, and on lactate turnover in fasted newborn dogs was studied at 0, 3, 6, 9, and 24 h of age. Maternal starvation reduced the birthweight of the puppies by 23% (232 ± 6 versus 300 ± 10 g). Fetal ketone body concentration (3.33 μmole/ml) paralleled the marked maternal(More)
Summary: Intrahepatic and intracerebral metabolic responses to neonatal fasting or enteric carbohydrate alimentation were investigated among newborn dogs. Pups were either fasted or given an intravenous glucose infusion (alimented) before an enteric feeding of physiologic quantities of either glucose or galactose. These pups were also compared to another(More)
A rapid and sensitive method for the analysis of plasma free fatty acids with glass capillary column gas-liquid chromatography and flame ionization detection is described. The plasma sample, together with n-pentadecanoic acid as an internal standard, was treated with 2,2-dimethoxypropane and hydrochloric acid. 2,2-Dimethoxypropane serves as water scavenger,(More)
Substrate-turnover relationships were determined in unanesthetized healthy fasted neonatal dogs during the first day of life. Pups were born at term by cesarean section to starved or control mothers. Pups born to starved mothers developed significantly lower blood glucose concentrations during neonatal fasting. In all pups, blood glucose concentrations(More)