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An in vitro system of T-dependent polyclonally activated B lymphocytes has been used to identify the cellular basis of immunosuppression induced by penicillamine. Suppression of immunoglobulin synthesis was dose dependent, and required the drug to be present at the initial stages of the culture. Coculture studies identified T helper cells as the primary(More)
Lymphocytes isolated from chronically-inflamed tissues were assessed for their ability to produce lymphokines without further stimulation in vitro. Cells were extracted from tissue obtained from 42 patients undergoing periodontal surgery. Cultures were set up in triplicate and supernatants collected after 48 h were assayed for interleukin-2 (IL-2) or(More)
Radioimmunoassay procedures for the quantitation of nanogram quantities of human immunoglobulin are described. The techniques have been successfully used to measure immunoglobulin secretion in culture supernatants by cultured human lymphocytes. Compared with previously published assays to assess lymphocyte function in vitro these procedures are simple,(More)
We assessed the immunoglobulin secretory capacity of circulating B lymphocytes in 9 patients with classical rheumatoid arthritis (RA) before and after treatment with D-penicillamine. Peripheral blood lymphocytes (PBL) from patients with RA spontaneously synthesized more IgG and IgA than normals. The secretory rate of rheumatoid PBL could not be induced by(More)
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