E De Bernardo

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To evaluate the functional aspects of B cell autonomy in human autoimmune thyroiditis we examined antigen-dependent antibody secretion using specific PFC assays for total immunoglobulin and antibodies to human thyroglobulin (hTg) and SRBC. A microculture system based on S. aureus and a macrophage-derived B cell differentiating factor provided the essential(More)
Investigations on cell-mediated immune release of 51Cr from the EL-4 ascitic lymphoma showed that daily rhythmic changes can be correlated with the dominance of certain cell types in the population. Cells in compartment 1--i.e., those representing true G0, early G1, S and M--are most sensitive to effector cell attack. During the establishment 96-hour(More)
Monocyte/macrophage function in patients with autoimmune thyroiditis was investigated by their presentation of two distinct antigens; sheep red blood cells (SRBC) and human thyroglobulin (hTg) using in vitro systems designed for antibody induction. Purified peripheral blood monocyte/macrophages were primed by prefeeding with antigen for 60 min at 37 degrees(More)
The 51Cr cytotoxicity assay was modified for ethylnitrosourea (ENU) and methylnitrosourea (MNU) induced rat neurogenic tumor cell lines. Investigations on "immune" lymphoid cytotoxicity showed little cytotoxicity above normal. Alteration in the level of lymphocytotoxicity was seen immediately after injection of a highly immunogenic tumor (EA-528). Natural(More)
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