E D Ponomarenko

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The method for the preparation of glycerol-treated models of the enterocytes with preserved membrane has been described. Using such a simplified model of the enterocyte suspension allows membrane digestion and enzyme properties to be studied. Moreover, preserving the important properties of the native isolated cells the glycerol-treated enterocytes have a(More)
BACKGROUND The condition of various organs can be characterized by the Na,K-ATPase activity therein. The present investigation was aimed at determining of Na,K-ATPase activity in erythrocytes from patients with chronic disturbances, resulting from viral infection and disturbances of non-viral origin. METHODS The functional activity of Na,K-ATPase was(More)
The mechanisms of Na, K-ATPase dysfunction in red blood cells of patients suffering from glomerulonephritis and renal failure were studied by means of "functional loads" of an enzyme, enabling not only the activity but also the routine of Na, K-ATPase to be determined. Evidence is provided for the presence of immunotoxic factor on the patients' red blood(More)
BACKGROUND In the cases of acute glomerulonephritis unfavourable outcome is related to increased blood concentration of IgM. We assumed that pathologic changes in glomerulonephritis might result from direct binding of IgM antibodies to Na, K-ATPase in the nephron channels. METHODS The study involved 30 patients suffering from glomerulonephritis. Na,(More)
BACKGROUND In some earlier work we have demonstrated that hypotonic mineral water can change the functional activity of Na, K-ATPase both in vitro and in vivo. The current investigation was aimed at measuring of the functional activity of ATPase in erythrocytes from blood samples of patients before and after treatment with the artificially mineralised water(More)
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