E. Benavente

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Karyotypic analysis of wheat lines with different genotypes for the homoeologous-pairing loci Ph1 and Ph2 was carried out by means of a genomic in situ hybridization method that allowed unequivocal identification of the A, B and D genomes. Chromosomal rearrangements mainly affecting the A and D genomes were found in all plants of allohexaploid wheat(More)
The metaphase I and anaphase I stages of meiosis of wheatxrye hybrids carrying theph1b mutation were analyzed by genomic in situ hybridization. This technique allows distinction between three different types of wheat-rye associations in metaphase I configurations as well as detection of wheat-rye recombinant chromosomes in anaphase I cells. The frequency of(More)
BACKGROUND AND AIMS Repetitive DNA sequences are thought to be involved in the formation of chromosomal rearrangements. The aim of this study was to analyse the distribution of microsatellite clusters in Aegilops biuncialis and Aegilops geniculata, and its relationship with the intergenomic translocations in these allotetraploid species, wild genetic(More)
Brachypodium distachyon (L.) P. Beauv. (2n = 2x = 10) is a small annual grass species where the existence of three different cytotypes (10, 20, and 30 chromosomes) has long been regarded as a case of autopolyploid series with x = 5. However, it has been demonstrated that the cytotypes assumed to be polyploids represent two separate Brachypodium species(More)
Meiosis of triticalextetraploid rye hybrids (genome constitution ABRRR) was analysed by genomic in situ hybridization (GISH) and C-banding. The results obtained reveal a considerable difference between these techniques with regard to their efficiency in detecting any type of pairing, either homologous or homoeologous. Thus the percentage of pollen mother(More)
 Wheat-wheat and wheat-rye homoeologous pairing at metaphase I and wheat-rye recombination at anaphase I were examined by genomic in situ hybridization (GISH) in wild-type (Ph1Ph2) and mutant ph1b and ph2b wheat×rye hybrids. The metaphase-I analysis revealed that the relative contribution of wheat-rye chromosome associations in ph2b wheat×rye was similar to(More)
Pairing competition between identical and homologous non-identical chromosomes is analysed in autotetraploid metaphase I cells of rye where one pair of identical partners bears an interstitial C-band in the long arm of chromosome 6R whereas the other pair of identical partners lacks such a C-band. This makes it possible to study pairing preferences in two(More)
Preferential chromosome association at metaphase I has been analyzed and compared in autotetraploid cells obtained by colchicine treatment of hybrid diploid rye plants with different degrees of chromosomal divergence between homologs. The tendency to identical over homologous, but not identical, pairing preferences detected when homologous partners are(More)
After two selfing generations of two different Triticum turgidum –Aegilops ovata amphiploids carrying the Ph1 gene, or lacking it (ph1c mutant), karyotypes of their offspring were scored by GISH (genomic in situ hybridization). On average, the chromosome number was lower than expected (56 chromosomes) on the basis of the parental constitutions (T. turgidum,(More)
Homoeologous metaphase I (MI) pairing of Triticum aestivum × Aegilops geniculata hybrids (2n = 5× = 35, ABDUgMg) has been examined by an in situ hybridization procedure permitting simultaneous discrimination of A, B, D and wild genomes. The seven D genome chromosomes (and their arms, except for 6D and 7D) plus some additional wheat chromosomes were also(More)