E. B. Wagenaar

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The protein synthesis inhibitor emetine was used to establish the times of synthesis of mitotic proteins, whose presence in the cell are essential in the mitotic processes of chromosome condensation, nuclear membrane breakdown, and possibly, chromosome alignment at metaphase. In embryos of the purple sea urchin, Strongylocentrotus purpuratus, protein(More)
The phosphorylation patterns of proteins were examined during the cell cycle of Chinese hamster ovary cells. This was accomplished by labeling synchronized cells at various times with [32P]orthophosphate and separating the proteins by both isoelectric focusing and nonequilibrium pH gradient two-dimensional gel electrophoresis. The most dramatic changes(More)
Cytological studies on telophase and early prophase in roottip cells of several plant species (Allium cepa, 2n=16; four Crepis species, including Crepis capillaris, 2n=6; Callitriche hermaphroditica, 2n=6; Nigella arvensis, 2n=12; Secale cereale, 2n=14) revealed that chromosome ends are attached two by two forming chains of chromosomes (interphase(More)
When partially synchronized root tip cells of Allium sativum L. (Garlic) were treated with cycloheximide during G2, some cells showed condensed chromosomes in arrested prophase nuclei. Other cells that were able to proceed into metaphase exhibited abnormal alignments of chromosomes at the metaphase plate, resulting in irregular anaphase and telophase(More)
Hydroxyapatite thermal elution chromatography was studied from an empirical standpoint. The dependence of elution temperature on elution buffer concentration was determined for various types of buffer, hydroxyapatite and nucleic acid. The results are analyzed in terms of the proper design and interpretation of thermal elution experiments. The potential for(More)
Palmelloids induced in the unicellular green alga Chlamydomonas eugametos by chloroplatinic acid treatment have been studied electron microscopically. Thin-sectioned specimens revealed the multilayer nature of the cell walls after second division within the palmelloid. Although synchrony in cell division is lost, to a certain degree, within the palmelloid,(More)