E B Vishnevskaia

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Polymerase chain reaction (PCR) was used to verify the tuberculous origin of L-forms isolated from clinical non-respiratory samples from patients with extrapulmonary tuberculosis. PCR was made by using cultured L-forms obtained from negative and positive cultures. PCR used a total of 60 cultured L-forms different in the morphology of colonies and the rate(More)
The detection rate and clinical and diagnostic values of L-forms of pathogens were determined in patients with pulmonary and extrapulmonary tuberculosis. Simultaneous culturing the specimens for typical and L forms of Mycobacterium tuberculosis (MBT) increased the number of positive results by isolating only L-forms by 10.3% in patients with pulmonary(More)
The polymerase chain reaction (PCR) and the ligase chain reaction (LCR) were evaluated and compared on 55 gynecological samples collected from women with active gynecological tuberculosis (Group 1), gynecological diseases other than tuberculosis (Group 2), and active tuberculosis elsewhere in the body without evidence for gynecological tuberculosis (Group(More)
A method has been developed for isolation of an antigen based on nonpolar lipids from M. tuberculosis cell walls, its specific and nonspecific fractions separated, and composition determined by thin-layer chromatography. Ninety-two blood sera of tuberculous patients and 50 ones from healthy donors were examined in enzyme immunoassay with this antigen. Use(More)
Inhibition of the polymerase chain reaction (PCR) by components of the analyzed specimen is an important problem in analysis of clinical material by this method. The inhibitory activity of clinical material can be decreased by additional purification of DNA by gel filtration on microcolumns. The method was developed using blood-containing specimens and(More)
PCR was used to study clinical materials from patients with extrapulmonary tuberculosis or diseases of nontuberculous etiology (renal tissue, scrapes of the endometrium and uterine cavity, bony fragments). It was shown that when the oligobacillary material analyzed, a concurrent study of some fragments of the sample largely enhanced the efficiency of PCR(More)
The aim of this work was to find the technique of enzyme immunoassay (EIA) of capillary blood lyophilized on a filter paper disk. The author has selected a buffer on the basis of egg yolk aqueous extract, that permits blocking of nonspecific binding in EIA but not preventing the specific antigen-antibody interactions. The results of analyses of capillary(More)
Three Russian commercial PCR diagnostic kits for detection of M. tuberculosis in clinical samples are compared. The kits were evaluated by sensitivity and convenience of analysis. The specificity and sensitivity of Russian diagnostic kits are not inferior to foreign analogs, but are not devoid of some drawbacks.
While testing samples by the polymerase chain reaction (PCR), a procedure for DNA isolation is advisable adapted for different types of materials; thorough DNA purification is of the greatest importance in testing blood-containing samples, deep sample lysis is of particular importance for osseous tissue samples. A differential approach to isolating DNA from(More)