E-A. Heinemeyer

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Guanosine 3'-diphosphate 5'-diphosphate (ppGpp) is rapidly degraded to guanosine 5'-diphosphate (ppG) and probably pyrophosphate by an enzyme present in the ribosomal fraction prepared from spoT+ strains of Escherichia coli. The ppGpp-degrading enzyme was released from the ribosomes during dissociation at low ionic strength. Ribosomes are not essential for(More)
Salmonella contamination of North Sea water was detected for the first time in 1988 in Germany during routine examinations of bathing areas. Since then, subsequent isolations along the coast have been reported regularly. To define the source of contamination, strains isolated from seawater and rivers were studied by molecular marker methods. Their(More)
Degradation of guanosine tetraphosphate (ppGpp) involves an enzyme associated with the ribosomal fraction from spoT+ strains of Escherichia coli. Double-label experiments with pp[3h]gpp, pp[3H]Gpp, or pp[3H]Gpp as substrate strongly suggest that ppG is the degradation product and that the enzyme releases two phosphates coordinately from the 3' position of(More)
The quantity of salmonella, fecal coliform bacteria and fecal streptococci was measured in 2003 water samples from North Sea coastal water, drainage canals, the lower River Ems and sewage works. The presence of salmonella did not correlate to an increasing presence of fecal indicator bacteria. In several cases more than 10(3) salmonella per litre were found(More)
Both substances, brilliant green and bile, inhibit the growth of gram-positive bacteria in culture media and selectively enrich gram-negative bacteria. Therefore, the brilliant green-lactose-bile broth (BRILA) and the BRLA broth supplemented with tryptophan and methyl-umbelliferyl-beta-D-glucuronide (BRILA-MUG) contain brilliant green as well as bile.(More)
Guanosine 5'-triphosphate 3'-diphosphate (pppGpp) and guanosine 5'-diphosphate 3'-diphosphate (ppGpp) are specifically degraded by a manganese-dependent pyrophosphorylase present in spoT+ but not in spoT- strains of Escherichia coli, indicating that the enzyme is the spoT gene product. The enzyme catalyzes the release of pyrophosphate from the 3' position(More)
Since the introduction and strict application of EC guidelines on the monitoring of bathing waters began in Lower Saxony in 1989, it has been possible for the first time to reach a comprehensive evaluation of the water quality on the North Sea coast and in the rivers and lakes of this state. The 7648 samples which were analysed in this period produced the(More)
In the monitoring of bathing waters according to EC Guideline 76/160 EWG, total coliform and faecal coliform bacteria have to be regularly determined by a quantitative method. This requirement can be fulfilled with sufficient reliability by the MPN method. Therefore, a procedure had to be developed which on the one hand is practical and not laborious, and(More)
In order to test the usefulness of BRILA-MUG (= Fluorocult) medium (Merck) for isolation and identification of total coliforms and faecal coliforms in surface water according to the EC guidelines for bathing waters a total of 969 strains of different Enterobacteriaceae and Vibrionaceae species was examined under different culture conditions. These included(More)