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Isolated, polarized, proximal tubule cells of Rana pipiens were voltage clamped and examined for both single-channel and whole cell currents. Barium-sensitive whole cell conductances were calculated from the difference in slopes of the current-voltage relations before and after 5 mM external barium. In 11 voltage-clamped cells with high K in the pipette(More)
We investigated the effect of beta-N-methylamino-L-alanine (L-BMAA) on input membrane resistance of leech Retzius nerve cells and the effect of the same substance on the membrane potential in the presence of 20 mM bicarbonate. Results of our experiments show that L-BMAA significantly reduces input membrane resistance on our model. This leads to the(More)
The role of neurotoxic non-protein amino acid beta-N-methylamino-L-alanine (L-BMAA) as a putative causative agent of Western pacific amyotrophic lateral sclerosis/Parkinsonism dementia complex (ALS/PDC) has recently been reinvigorated. In view of this data we have investigated the strength and mechanism of effect of L-BMAA in presence of 20 mmol/L(More)
The effects of Lathyrus sativus neurotoxin were studied on the cell membrane potential and cellular cation composition in Retzius nerve cells of the leech Haemopis sanguisuga, with ion-selective microelectrodes using liquid ion-exchangers. Bath application of 10(-4) mol/l Lathyrus sativus neurotoxin for 3 min depolarized the cell membrane potential and(More)
1. A significant drop in effective input resistance of the free membrane and an increase in effective coupling resistance in acid Ca2+ Ringer (complete replacement of Na+ with Ca2+, pH 4) compared to control medium has been obtained in leech Retzius neurons. 2. In neutral Ca2+ Ringer (pH 7.2), effective input resistance increased while effective coupling(More)
In this paper we examine the effects of L-aspartate, L-glutamate, and beta-N-oxalylamino-L-alanine (Lathyrus toxin) on Retzius nerve cells of the leech Haemopis sanguisuga. The goal was to compare the electrophysiological effects of endogenous vs. exogenous amino acids, known as potent neurotoxins, through the mechanism ofexcitotoxicity. We used classical(More)
In the present study we have examined effects of ethanol and magnesium on Ni(2+)-induced bursting of leech Retzius cells. Saline with 3 mmol/l NiCl2 induced spontaneous bursting activity, characterized by rapid depolarizations to a plateau level during which bursts of action potentials occurred. To test for the mechanism of bursting initiation external Na+(More)
The aim of this study was to evaluate KCNQ1 K+ channel expression in the frog kidney of Rana esculenta. KCNQ1 K+ channel, also known as KvLQT1, is the pore forming a-subunit of the IKs K+ channel, a delayed rectifier voltage-gated K+ channel, which has an important role in water and salt transport in the kidney and gastrointestinal tract. The expression of(More)
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