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Human and animal fungal pathogens are a growing threat worldwide leading to emerging infections and creating new risks for established ones. There is a growing need for a rapid and accurate identification of pathogens to enable early diagnosis and targeted antifungal therapy. Morphological and biochemical identification methods are time-consuming and(More)
Scedosporium species show decreased susceptibility to the majority of systemic antifungal drugs. Acquired resistance is likely to disseminate differentially with the mode of exchange of genetic material between lineages. Inter- and intraspecific diversities of Scedosporium species were analyzed for three partitions (rDNA internal transcribed spacer gene(More)
DNA phylogenetic comparisons have shown that morphology-based species recognition often underestimates fungal diversity. Therefore, the need for accurate DNA sequence data, tied to both correct taxonomic names and clearly annotated specimen data, has never been greater. Furthermore, the growing number of molecular ecology and microbiome projects using(More)
The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS rDNA as primary barcode. Amplification efficiencies of 14 (partially) universal primer pairs targeting eight genetic markers were tested across > 1 500 species (1 931 strains or(More)
The BioloMICS software is briefly described in this application note. BioloMICS is a tool allowing specialized and scientific biological databases to be created to fit the specific needs of researchers working on any organisms (from viruses, bacteria, fungi, plants, insects to animals) by abroad base of users such as taxonomists, ecologists, human, animal(More)
MycoBank, a registration system for fungi established in 2004 to capture all taxonomic novelties, acts as a coordination hub between repositories such as Index Fungorum and Fungal Names. Since January 2013, registration of fungal names is a mandatory requirement for valid publication under the International Code of Nomenclature for algae, fungi and plants(More)
DNA barcoding is a global initiative for species identification through sequencing of short DNA sequence markers. Sequences of two loci, ITS and LSU, were generated as barcode data for all (ca. 9k) yeast strains included in the CBS collection, originally assigned to ca. 2 000 species. Taxonomic sequence validation turned out to be the most severe bottleneck(More)
With the availability of newer and cheaper sequencing methods, genomic data are being generated at an increasingly fast pace. In spite of the high degree of complexity of currently available search routines, the massive number of sequences available virtually prohibits quick and correct identification of large groups of sequences sharing common traits.(More)
A simple low-cost system for detection of polycyclic aromatic hydrocarbon (PAH) in solution based on multiphoton ionization configuration is designed using a circulating ionization cell of 0.1 × 2 × 5 mm dimension with quartz optical window. Fourth harmonic emission of Nd:YAG laser (266 nm, 6 ns, 10 Hz, and 2 mJ) and second harmonic generation of(More)
TÓM TẮT Thái độ một khái niệm quan trọng trong phạm trù cảm xúc – có một chỗ đứng quan trọng trong lĩnh vực nghiên cứu giáo dục khoa học thế giới. Tuy nhiên ở Việt Nam, những nghiên cứu về thái độ của học sinh đối với các môn khoa học nói chung và Hóa học nói riêng vẫn chưa phổ biến. Bài viết này bàn về việc xây dựng một thang đo có giá trị và đáng tin cậy(More)