Duane B. Huggett

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Attempts to better understand causal factors affecting estrogenicity in municipal wastewater have primarily focused on analytical evaluation of specific chemical estrogens and the use of estrogen receptor (ER) based in vitro assays. To compare analytical,in vitro, and in vivo assays for estrogenicity, wastewater from four New York and one Texas municipal(More)
In this study, an in silico genome-scale metabolic model of steroidogenesis was used to investigate the effects of hypoxic stress on steroid hormone productions in fish. Adult female fathead minnows (Pimephales promelas) were exposed to hypoxia for 7 days with fish sub-sampled on days 1, 3 and 7 of exposure. At each time point, selected steroid enzyme gene(More)
Webb, Veronica Fine. Investigation of cryopreservation methods for adherent nerve cell networks in vitro Cryopreservation in suspension is commonplace for a variety of cell types. However, cryopreservation of adherent cells has achieved limited success. This research aimed to cryopreserve adherent nerve cell networks in vitro in a manner that preserved(More)
Genome-scale metabolic models associate genes and transcript enzymes involved in biochemical reactions. We present a genome-scale stoichiometric reconstruction of the zebrafish (<i>Danio rerio</i>) steroidogenic network and apply linear programming methods to investigate the association between steroidogenic gene expression and network redundancy required(More)
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