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An efficient transformation method for Bacillus subtilis DB104
Bacillus subtilis strains are used for extracellular expression of enzymes (i.e., proteases, lipases, and cellulases) which are often engineered by directed evolution for industrial applications. B.Expand
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Redirecting catalysis from proteolysis to perhydrolysis in subtilisin Carlsberg.
Enzyme promiscuity describes the ability of biocatalysts to catalyze conversions beyond their natural reactions. Enzyme engineering to promote side reactions is attractive for synthetic andExpand
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Rationalizing perhydrolase activity of aryl-esterase and subtilisin Carlsberg mutants by molecular dynamics simulations of the second tetrahedral intermediate state
The perhydrolysis reaction in hydrolases is an important example of catalytic promiscuity and has many potential industrial applications. The mechanisms of perhydrolase activity of a subtilisinExpand
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Fluorescent Assay for Directed Evolution of Perhydrolases
Directed evolution offers opportunities to improve promiscuous activities of hydrolases in rounds of diversity generation and high-throughput screening. In this article, we developed and validated aExpand
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Primordial emergence of a nucleic acid binding protein via phase separation and statistical ornithine to arginine conversion
De novo emergence, and emergence of the earliest proteins specifically, demands a transition from disordered polypeptides into structured proteins with well-defined functions. However, can peptidesExpand
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Reengineering of subtilisin Carlsberg for oxidative resistance
Abstract Mild bleaching conditions by in situ production of hydrogen peroxide or peroxycarboxylic acid is attractive for pulp, textile, and cosmetics industries. The enzymatic generation of chemicalExpand
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