Dr.E. Ramachandra Rao

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Enterohemorrhagic Escherichia coli O157:H7, an emerging food-borne pathogen, has been implicated in several outbreaks in the US. Ruminants, including cattle, sheep and deer are reservoirs of E. coli O157:H7 and fecal shedding of the pathogen forms the vehicle of entry into the human food chain. We studied the ef®cacy of Lactobacillus acidophilus,(More)
Field resistance to Bacillus sphaericus was observed in a population of Culex quinquefasciatus in Kochi, India, exposed to 35 rounds of spraying with a formulation of B. sphaericus 1593 M over a 2-year period. Larvae from the sprayed area gave LC50 and LC90 values that were 146 and 180 times greater than corresponding values for a susceptible strain from an(More)
Crude neem products have earlier shown considerable promise for control of culicine mosquito vectors in rice fields as a by-product of their agricultural use as fertilizers, but suffer from disadvantages of bulkiness and lack of stability in storage. Relatively stable lipid-rich fractions of neem were shown to be as effective as good-quality crude neem(More)
Bacillus sphaericus 1593M resistant larvae of Culex quinquefasciatus were reared in the laboratory since 1995. Resistance in the larvae was monitored by subjecting selection pressure using B. sphaericus 1593M at every generation. Bioassays were conducted with different strains of B. sphaericus (Bs 2297, Bs 2362 and Bs IAB 59) and confirmed cross-resistance(More)
A microgel droplet formulation of Bacillus sphaericus 1593M (Biocide-S) was field tested at two different doses viz, 0.22 and 0.43 g/m2 in casuarina pits against a malarial vector Anopheles (Cellia) culicifacies Giles, and An. (Cel.) subpictus Grassi in coconut garden pits in the coastal areas of South India. Both doses significantly reduced the abundance(More)
We studied the cross-resistance to three highly toxic Bacillus sphaericus strains, IAB-59 (serotype H6), IAB-881 (serotype H3), and IAB-872 (serotype H48), of four colonies of the Culex pipiens complex resistant to B. sphaericus 2362 and 1593, both of which are serotype H5a5b strains. Two field-selected highly resistant colonies originating from India(More)
Applications of neem, Azadirachta indica (Meliaceae), to rice-fields were evaluated with the dual objective of controlling the culicine mosquito vectors of Japanese encephalitis virus (JEV) and enhancing the grain yield. Since crude neem products deteriorate under improper storage conditions, a laboratory bioassay was developed to screen neem cake powder(More)