Douglas P. Middaugh

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Weathered Alaska North Slope crude oil (ANS 521) was subjected to biodegradation in vigorously stirred incubations for 14 days at 15 +/- 1 degrees C in 20/1000 salinity sterilized seawater, amended with nutrients and inoculated with a hydrocarbon-degrading microorganism (EI2V) isolated from an oil-contaminated beach in Prince William Sound, Alaska. A total(More)
Weathered Alaska North Slope crude oil (ANS 521) was stirred for 2 and 14 days in 20 per thousand salinity sterile seawater or for 14 days in seawater with nutrients and a group of three (GO3) microorganisms from Prince William Sound, Alaska, that were capable of biodegrading hydrocarbons. A total of 0.65 and 0.69 mg/L water soluble fraction (WSF) of(More)
A Gram-negative bacterium, Pseudomonas sp. strain SR3, was isolated from soil at a former wood treatment plant in north central Florida. The ability of this bacterium to degrade pentachlorophenol (PCP) was confirmed by growing cells in a basal salts medium in which PCP was the only source of carbon and energy. Degradation from a measured concentration of(More)
Embryonic inland silversides, Meinida beryllina, were exposed to neutral, water-soluble fractions (WSFs) resulting from microbial degradation of artificially weathered Alaska North Slope (ANS) crude oil. Three individual microbes obtained from Prince William Sound, Alaska, and designated Phe#6 (enriched on phenanthrene), Hexaco#2 (enriched on the(More)
Spawning patterns of inland silversides, Menidia beryllina, and tidewater silversides, Menidia peninsulae, were examined in the laboratory under several combinations of ‘tidal’ and diel light cycle cues. M. beryllina showed a high frequency of spawning throughout the day when held under constant conditions (24L: OD, current velocity 8 cm sec−1) and when(More)
To determine whether a 7- to 10-d embryo toxicity/teratogenicity test with the inland silverside fish, Menidia beryllina, is a sensitive indicator for evaluation of bleached kraft mill effluents, we compared this test with the Microtox(R) 15-min acute toxicity test and the Ceriodaphnia dubia 7-d chronic toxicity test. Water samples used in each test were(More)
Sand fiddler crabs, Uca panacea, were exposed in laboratory habitats to measured concentrations of ULV-grade fenthion via simulated aerial spray at 5% and 50% of field rate application of 6-12 mg fenthion/m(2) (0.05-0.10 lbs fenthion/acre). Two habitats served as controls, and two habitats served as replicates for each of the test exposures. The aerial(More)
The spawning behavior of the Atlantic silverside,Menidia menidia, was studied at two sites on the North Edisto River estuary in South Carolina. Prespawning schools moved back and forth along the shoreline as the time of high tide approached. Spawning runs took place in the upper intertidal zone at high tide. Spawning fish deposited their eggs on three types(More)