Douglas C. Knipple

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The Krüppel (Kr) locus is a member of the 'gap' class of segmentation genes of Drosophila melanogaster. Mutations at the Kr locus cause the deletion of contiguous segments from the embryonic body pattern. We have elucidated the spatial and temporal characteristics of Kr gene expression during early embryo development, the localization of cytoplasmic Kr+(More)
Voltage-sensitive sodium channels encoded by a full-length cDNA corresponding to the Vssc1 gene of the house fly (Musca domestica) were expressed in Xenopus laevis oocytes either alone or in combination with the tipE gene product of Drosophila melanogaster and were characterized by two-electrode voltage clamp. Vssc1 cRNA alone produced very small (50-150(More)
The demonstration that a specific messenger RNA can be functionally inactivated in vivo by hybridization to complementary polynucleotide sequences suggests a direct approach to the study of gene function in cells of higher organisms. The experiments described here were designed to inhibit, by complementary RNA sequences, a specific gene function affecting(More)
Sequence analysis of cDNA clones amplified by PCR from house fly ( Musca domestica L.) Vssc1 voltage-sensitive sodium channel alpha subunit transcript templates identified 11 putative alternatively spliced exons. Nine of these corresponded to the 7 optional exons (designated a, b, e, f, h, i, and j) and 2 mutually exclusive exons (designated c/d) identified(More)
1. In assays of 36Cl- uptake into mouse brain vesicles, 100 microM GABA markedly increased both the initial rate of 36Cl- uptake and the total amount of chloride taken up over a 120-sec incubation period. Specific GABA-dependent 36Cl- uptake (the difference between total and background uptake) was essentially complete within 15 sec of incubation. 2.(More)
Fields of Research Specialization Mode of action and neurotoxicity of synthetic insecticides, natural toxins, and drugs Mechanisms of resistance to insecticides and nematicides Expression and function of membrane transporters for neurotransmitters (2008) Nematicidal activity of anion transport blockers against Meloidogyne incognita, Ceanorhabditis elegans,(More)
Mutations of the maternal effect locus pelle (pll) cause dorsalized Drosophila embryos. In extreme mutants, the embryo develops into a long hollow tube of dorsal cuticular structures with no sign of ventral pattern elements. Injection of wild-type cytoplasm or poly(A)+RNA into mutant pll embryos partially restores the normal pattern. Rescuing activity is(More)
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