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Do the coats on vesicles budded from the Golgi apparatus actually cause the budding, or do they simply coat buds (Fig. 1)? One view (the membrane-mediated budding hypothesis) is that budding is an intrinsic property of Golgi membranes not requiring extrinsic coat proteins. Assembly of coats from dispersed subunits is super-imposed upon the intrinsic budding(More)
Transport vesicles derived from the Golgi apparatus are thought to mediate biosynthetic transport across the Golgi stack. These vesicles are surrounded by a protein coat whose principal constituents are coatomer (a complex of seven distinct subunits or COPs) and ADP-ribosylation factor (ARF, an N-myristylated small GTP-binding protein). The coat proteins of(More)
Coatomer, a complex of seven proteins, appears to be the precursor of the coat structure of non-clathrin-coated Golgi-derived vesicles. Another component of this vesicle coat is the cytosolic protein ADP-ribosylation factor (ARF). Like coatomer, ARF appears to reversibly associate with Golgi membranes. We now report that ARF is required for coatomer binding(More)
ADP-ribosylation factor (ARF) is a small molecular weight GTP-binding protein (20 kD) and has been implicated in vesicular protein transport. The guanine nucleotide, bound to ARF protein is believed to modulate the activity of ARF but the mechanism of action remains elusive. We have previously reported that ARF binds to Golgi membranes after Brefeldin(More)
A novel alpha-chemokine, designated KS1, was identified from an EST database of a murine immature keratinocyte cDNA library. The EST has 94% similarity to a recently cloned human gene, BRAK, that has no demonstrated function. Northern analysis of mouse and human genes showed detectable mRNA in brain, intestine, muscle and kidney. Tumour panel blots showed(More)
Availability of a wide variety of Web services over the Internet offers opportunities of providing new value added services built by composing them out of existing ones. Service composition poses a number of challenges. A composite service can be very complex in structure, containing many temporal and data-flow dependencies between their constituent(More)
Web Services technology is becoming the de-facto standard for integration of legacy applications within an organisation and for cross organisation enterprise application integration. Much work has gone into specifying Web Services functionality using workflow like techniques and production of software to enact such descriptions. However, most of this(More)
Web Services technology is becoming the de-facto standard for integration of legacy applications within an organisation and for cross organisation enterprise application integration (B2BEAI). Much work has gone into specifying Web Services functionality using workflow like techniques (BPEL4WS, WSCI, WSFL, XLANG) and production of software to enact such(More)