Learn More
In the present study, we determined characteristic repeat distances of the photosynthetic membranes in living cyanobacterial and eukaryotic algal cells, and in intact thylakoid membranes isolated from higher plants with time-resolved small-angle neutron scattering. This non-invasive technique reveals light-induced reversible reorganizations in the(More)
We studied the periodicity of the multilamellar membrane system of granal chloroplasts in different isolated plant thylakoid membranes, using different suspension media, as well as on different detached leaves and isolated protoplasts-using small-angle neutron scattering. Freshly isolated thylakoid membranes suspended in isotonic or hypertonic media,(More)
We demonstrate the power of time-resolved small-angle neutron scattering experiments for the investigation of the structure and structural reorganizations of multilamellar photosynthetic membranes. In addition to briefly summarizing our results on thylakoid membranes isolated from higher plants and in unicellular organisms, we discuss the advantages and(More)
We have investigated the circular dichroism spectral transients associated with the light-induced reversible reorganizations in chirally organized macrodomains of pea thylakoid membranes and loosely stacked lamellar aggregates of the main chlorophyll a/b light harvesting complexes (LHCII) isolated from the same membranes. These reorganizations have earlier(More)
By use of small-angle X-ray scattering it is shown that addition of alkali halide salts in small amounts (0-200 mM) shifts the repeat spacing in multilamellar DC13PC vesicles and alters the anomalous swelling behaviour close to the main transition. Both effects follow the Hofmeister series of the ions. We suggest that the shift of repeat spacing can be(More)
The multilamellar organization of freshly isolated spinach and pea chloroplast thylakoid membranes was studied using small-angle neutron scattering. A broad peak at ~0.02Å(-1) is ascribed to diffraction from domains of ordered, unappressed stroma lamellae, revealing a repeat distance of 294ű7Å in spinach and 345ű11Å in pea. The peak position and hence the(More)
We have investigated the structural changes in thin films of lamellar poly(styrene-b-butadiene) diblock copolymers during treatment with saturated cyclohexane vapor, a solvent slightly selective for polybutadiene. Using real-time, in-situ grazing-incidence small-angle X-ray scattering (GISAXS), the swelling and the rearrangement of the lamellae were(More)
The reorientation of lamellae and the dependence of the lamellar spacing, D lam , on polymer volume fraction, ϕ P , D lam ∝ ϕ P −β , in diblock copolymer thin films during solvent vapor annealing (SVA) are examined by combining white light interferometry (WLI) and grazing-incidence small-angle X-ray scattering (GISAXS). A thin film of lamellae-forming(More)
The nanostructures of thin films spin-coated from binary blends of compositionally symmetric polystyrene-b-polybutadiene (PS-b-PB) diblock copolymer having different molar masses are investigated by means of atomic force microscopy (AFM) and grazing-incidence small-angle X-ray scattering (GISAXS) after spin-coating and after subsequent solvent vapor(More)
Each topic is introduced by a motivational or tutorial talk on a selected scientific achievement, in such a way that all participants can gain a broader sense of the benefits of the various approaches described. Basic information on the complementary properties of the different types of techniques, their data interpretation, as well as information on recent(More)