• Publications
  • Influence
Characterization and engineering of 3-ketosteroid-△1-dehydrogenase and 3-ketosteroid-9α-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9α-hydroxy-4-androstene-3,17-dione through the
3-Ketosteroid-△(1)-dehydrogenase (KstD) is a key enzyme involved in the microbial catabolism of sterols. Here, three homologues of KstD were characterized from Mycobacterium neoaurum ATCC 25795,Expand
  • 58
  • 9
Ganoderic acid T from Ganoderma lucidum mycelia induces mitochondria mediated apoptosis in lung cancer cells.
Ganoderma lucidum is a well-known traditional Chinese medicinal herb containing many bioactive compounds. Ganoderic acid T (GA-T), which is a lanostane triterpenoid purified from methanol extract ofExpand
  • 198
  • 8
  • PDF
Phytochemical profiles of different mulberry (Morus sp.) species from China.
Mulberry is rich in alkaloids, polyphenols, flavonoids, and anthocyanins, which have been suggested to be responsible for health benefits. The concentrations of 1-deoxynojirimycin (DNJ), resveratrol,Expand
  • 135
  • 8
Inactivation and Augmentation of the Primary 3-Ketosteroid-Δ1- Dehydrogenase in Mycobacterium neoaurum NwIB-01: Biotransformation of Soybean Phytosterols to 4-Androstene- 3,17-Dione or
ABSTRACT 3-Ketosteroid-Δ1-dehydrogenase, KsdDM, was identified by targeted gene disruption and augmentation from Mycobacterium neoaurum NwIB-01, a newly isolated strain. The difficulty of separatingExpand
  • 53
  • 6
Unraveling and engineering the production of 23,24-bisnorcholenic steroids in sterol metabolism
The catabolism of sterols in mycobacteria is highly important due to its close relevance in the pathogenesis of pathogenic strains and the biotechnological applications of nonpathogenic strains forExpand
  • 27
  • 6
ING4 induces G2/M cell cycle arrest and enhances the chemosensitivity to DNA‐damage agents in HepG2 cells
The known members of inhibitor of growth (ING) gene family are considered as candidate tumor suppressor genes. ING4, a novel member of ING family, is recently reported to interact with tumorExpand
  • 105
  • 5
Nuclear localization signal of ING4 plays a key role in its binding to p53.
ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53 and negatively regulate the cell growth with significant G2/M arrest of cell cycle in HepG2 cellsExpand
  • 67
  • 5
Enhanced acetoin production by Serratia marcescens H32 with expression of a water-forming NADH oxidase.
Cofactor engineering was employed to enhance production of acetoin by Serratia marcescens H32. 2,3-Butanediol was a major byproduct of acetoin fermentation by S. marcescens H32. In order to decreaseExpand
  • 74
  • 5
Determination of the amino acid sequence in a cyclic lipopeptide using MS with DHT mechanism.
A TOF MS/MS method to directly determine the amino acid sequence in a cyclic lipopeptide without its hydrolysis is described. The fragments of the peptide and the hydrocarbon chains were identifiedExpand
  • 51
  • 5
Identification and engineering of cholesterol oxidases involved in the initial step of sterols catabolism in Mycobacterium neoaurum.
Mycobacteria have been modified to transform sterols to produce valuable steroids. Here, we demonstrated that the oxidation of sterols to sterones is a rate-limiting step in the catabolic pathway ofExpand
  • 52
  • 4
  • PDF