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A proteomics approach to understanding protein ubiquitination
TLDR
A proteomics approach to enrich, recover, and identify ubiquitin conjugates from Saccharomyces cerevisiae lysate provides a general tool for the large-scale analysis and characterization of protein ubiquitination. Expand
Quantitative Proteomics Reveals the Function of Unconventional Ubiquitin Chains in Proteasomal Degradation
TLDR
It is reported that the unconventional linkages are abundant in vivo and that all non-K63 linkages may target proteins for degradation, and that unconventional polyubiquitin chains are critical for ubiquitin-proteasome system function. Expand
Semiquantitative Proteomic Analysis of Rat Forebrain Postsynaptic Density Fractions by Mass Spectrometry*
TLDR
A comprehensive analysis of purified PSD fractions by liquid chromatography coupled with tandem mass spectrometry reveals crucial information about molecular abundance as well as molecular diversity in the PSD, and provides a basis for further studies on the molecular mechanisms of synaptic function and plasticity. Expand
Autophosphorylated CaMKIIα Acts as a Scaffold to Recruit Proteasomes to Dendritic Spines
TLDR
It is reported that CaMKIIalpha-an abundant postsynaptic protein kinase-mediates the activity-dependent recruitment of proteasomes to dendritic spines in hippocampal neurons, revealing a scaffolding role of post Synaptic CaMKIalpha in activity- dependent proteasome redistribution, which is commensurate with the great abundance of CaMK IIalpha in synapses. Expand
Human LSD2/KDM1b/AOF1 regulates gene transcription by modulating intragenic H3K4me2 methylation.
TLDR
Characterization of the LSD2 complex reveals that LSD2 forms active complexes with euchromatic histone methyltransferases G9a and NSD3 as well as cellular factors involved in transcription elongation, providing a possible molecular mechanism linking LSD2 to transcriptional regulation after initiation. Expand
Relative and Absolute Quantification of Postsynaptic Density Proteome Isolated from Rat Forebrain and Cerebellum*S
TLDR
This study delineates a crucial molecular difference between forebrain and cerebellar PSDs and provides a quantitative framework for measuring the molecular stoichiometry of the PSD, and demonstrates that the absolute quantification method is well suited for targeted quantitative proteomic analysis. Expand
Analysis of the Large Inactive P-TEFb Complex Indicates That It Contains One 7SK Molecule, a Dimer of HEXIM1 or HEXIM2, and Two P-TEFb Molecules Containing Cdk9 Phosphorylated at Threonine 186*
TLDR
A model illustrates what is currently known about how HEXIM proteins, 7SK, and P-TEFb assemble to maintain an activated kinase in a readily available, but inactive form. Expand
Proteomic Characterization of Postmortem Amyloid Plaques Isolated by Laser Capture Microdissection*[boxs]
TLDR
A comprehensive proteomic analysis of senile plaques from postmortem AD brain tissues revealed for the first time the sub-proteome of amyloid plaques that is important for further studies on disease biomarker identification and molecular mechanisms of AD pathogenesis. Expand
A novel transferrin/TfR2-mediated mitochondrial iron transport system is disrupted in Parkinson's disease
TLDR
A novel transferrin/transferrin receptor 2 (Tf/TfR2)-mediated iron transport pathway in mitochondria of SN dopamine neurons is reported, which has a previously uncharacterized mitochondrial targeting sequence that is sufficient to import the protein into these organelles. Expand
Prolyl 4-hydroxylation regulates Argonaute 2 stability
TLDR
Hydroxylation is identified as a post-translational modification important for Ago2 stability and effective RNA interference. Expand
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