Dongling Wu

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OBJECTIVE To study the chemical constituents from the root barks of Morus atropurpurea. METHOD The chemical constituents from the 70% ethanol extract of M. atropurpurea were isolated and purified by column chromatographic methods. Their structures were identified by physico-chemical properties as well as spectral data. RESULT Fifteen compounds were(More)
OBJECTIVE To investigate the effects of D-galactose (D-gal) on aging of rat marrow mesenchymal stem cells (MSCs) and its mechanism. METHODS MSCs isolated from young (7 d) SD rats were randomly divided into four groups:control group, 1g/L, 10g/L and 50g/L D-gal treatment groups. In control group MSCs were cultured in DMEM containing 10% FBS for 48 h. In(More)
OBJECTIVE A19-deltaVirB12 deletion mutant of Brucella abortus was constructed by using homologous recombination technology. BALB/c mice were vaccinated intraperitoneally with the mutant to evaluate protective efficacy against Brucella abortus 2308 challenge. METHOD A SacB gene was amplified by PCR from pIB279 plasmid. The sequences upstream and downstream(More)
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