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We report the observation of Sommerfeld-Brillouin optical precursors generated from a long square-modulated laser pulse propagating through a cold atomic ensemble with electromagnetically induced transparency. The optical depth (alpha(0)L) of the medium can be varied from 0 up to 50. We demonstrated that the step-on rising and step-off falling edges(More)
In this study, we optimized a restriction-ligation-free (RLF) method to save time and cost of constructing multiple plasmids with the same gene insert, and examined the efficacy of RLF on high-throughput multi-plasmid cloning. This method utilizes the precise DNA repair and recombination systems within Escherichia coli, which allows to bypass the in vitro(More)
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