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were first optimized for high level protein expression in E. coli. They were then synthesized using DNA oligo based, PCR gene assembling method. Poly-arginine tag plus a linker sequence, i.e. ESGGGGSPGRRRRRRRRRRR, was added to each protein C-terminal. The final DNA fragment was flanked with Nde I and Xho I sites, and inserted into pET41a expression vector(More)
Embryonic stem cells (ESCs) comprise at least two populations of cells with divergent states of pluripotency. Here, we show that epiblast stem cells (EpiSCs) also comprise two distinct cell populations that can be distinguished by the expression of a specific Oct4-GFP marker. These two subpopulations, Oct4-GFP positive and negative EpiSCs, are capable of(More)
Reprogramming of somatic cells is a valuable tool to understand the mechanisms of regaining pluripotency and further opens up the possibility of generating patient-specific pluripotent stem cells. Reprogramming of mouse and human somatic cells into pluripotent stem cells, designated as induced pluripotent stem (iPS) cells, has been possible with the(More)
Recognizing human action in non-instrumented video is a challenging task not only because of the variability produced by general scene factors like illumination, background, occlusion or intra-class variability, but also because of subtle behavioral patterns among interacting people or between people and objects in images. To improve recognition, a system(More)
—In order to realize the Smart Grid vision, it is necessary to have guaranteed Quality of Service (QoS) for the communication and networking technology used in various stages of the Smart Grid, ranging from power generation, transmission, distribution, to the customer applications. The low cost wireless protocols such as Zigbee (using IEEE 802.15.4 defined(More)
The overall biological role and clinical significance of long non-coding RNA H19 in colorectal cancer (CRC) remain largely unknown. Here, we firstly report that the lncRNA H19 recruits eIF4A3 and promotes the CRC cell proliferation. We observed higher expression of H19 was significantly correlated with tumor differentiation and advanced TNM stage in a(More)
Reprogramming of somatic cells achieved by combination of the four transcription factors Oct4, Sox2, Klf4, and c-Myc has very low efficiency. To increase the reprogramming efficiency and better understand the process, we sought to identify factors that mediate reprogramming with higher efficiency. We established an assay to screen nuclear fractions from(More)
BACKGROUND Oxidative stress is known to play an important role in the pathology of traumatic brain injury. Mitochondria are thought to be the major source of the damaging reactive oxygen species (ROS) following TBI. However, recent work has revealed that the membrane, via the enzyme NADPH oxidase can also generate the superoxide radical (O(2)(-)), and(More)
BACKGROUND Recent work by our laboratory and others has implicated NADPH oxidase as having an important role in reactive oxygen species (ROS) generation and neuronal damage following cerebral ischemia, although the mechanisms controlling NADPH oxidase in the brain remain poorly understood. The purpose of the current study was to examine the regulatory and(More)
Conrad et al. have generated human adult germline stem cells (haGSCs) from human testicular tissue, which they claim have similar pluripotent properties to human embryonic stem cells (hESCs). Here we investigate the pluripotency of haGSCs by using global gene-expression analysis based on their gene array data and comparing the expression of pluripotency(More)