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- Dong Gon Yu, Howard M. Ellis, Eui Cheol Lee, Nancy A. Jenkins, Neal G. Copeland, Donald L. Court
- Proceedings of the National Academy of Sciences…
- 2000
A recombination system has been developed for efficient chromosome engineering in Escherichia coli by using electroporated linear DNA. A defective lambda prophage supplies functions that protect and… (More)
- Lisa Timmons, Donald L. Court, A. Fire
- Gene
- 2001
Genetic interference mediated by double-stranded RNA (RNAi) has been a valuable tool in the analysis of gene function in Caenorhabditis elegans. Here we report an efficient induction of RNAi using… (More)
- Søren Warming, Nina Costantino, Donald L. Court, Nancy A. Jenkins, Neal G. Copeland
- Nucleic acids research
- 2005
Recombineering allows DNA cloned in Escherichia coli to be modified via lambda (lambda) Red-mediated homologous recombination, obviating the need for restriction enzymes and DNA ligases to modify… (More)
- Jaroslaw Blaszczyk, Joseph E. Tropea, +4 authors Xinhua Ji
- Structure
- 2001
BACKGROUND
Aquifex aeolicus Ribonuclease III (Aa-RNase III) belongs to the family of Mg(2+)-dependent endonucleases that show specificity for double-stranded RNA (dsRNA). RNase III is conserved in… (More)
- Bradford S Powell, Donald L. Court, +6 authors Jonathan Reizer
- The Journal of biological chemistry
- 1995
Two rpoN-linked delta Tn10-kan insertions suppress the conditionally lethal erats allele. One truncates rpoN while the second disrupts another gene (ptsN) in the rpoN operon and does not affect… (More)
- Neal G. Copeland, Nancy A. Jenkins, Donald L. Court
- Nature Reviews Genetics
- 2001
Highly efficient phage-based Escherichia coli homologous recombination systems have recently been developed that enable genomic DNA in bacterial artificial chromosomes to be modified and subcloned,… (More)
- Nina Costantino, Donald L. Court
- Proceedings of the National Academy of Sciences…
- 2003
Homologous recombination can be used to generate recombinants on episomes or directly on the Escherichia coli chromosome with PCR products or synthetic single-stranded DNA (ssDNA) oligonucleotides… (More)
- Hilary M. Ellis, Deyue Yu, T DiTizio, Donald L. Court
- Proceedings of the National Academy of Sciences…
- 2001
Homologous DNA recombination is a fundamental, regenerative process within living organisms. However, in most organisms, homologous recombination is a rare event, requiring a complex set of reactions… (More)
Members of the ribonuclease III (RNase III) family are double-stranded RNA (dsRNA) specific endoribonucleases characterized by a signature motif in their active centers and a two-base 3' overhang in… (More)
- Amos B. Oppenheim, Oren Kobiler, Joel Stavans, Donald L. Court, Sankar 8 Adhya
- Annual review of genetics
- 2005
The lysis-lysogeny decision of bacteriophage lambda (lambda) is a paradigm for developmental genetic networks. There are three key features, which characterize the network. First, after infection of… (More)