Donald J. Donaldson

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To study the interaction of migrating newt epidermal cells with purified extracellular matrix (ECM) molecules we have developed an in vitro migration assay using pieces of newt skin explanted onto culture dishes coated with various ECM molecules and cultured for 18 h in defined serum-free medium. Newt epidermal cells migrate out from explants placed on(More)
Epidermal closure of skin wounds on newt limb explants was inhibited to equal degrees by cytochalasins B,D and dihydrocytochalasin B (H2CB). The cytochalasin solvent, dimethylsulfoxide (DMSO), had no effect on migration at the low concentration present in the cytochalasin and control solutions. However, a 5% DMSO solution completely blocked mobility. Wounds(More)
It has been hypothesized that there are differences between membrane-associated glycoconjugates of wounded (migrating) epithelium and those of nonwounded (stationary) epithelium. To test this hypothesis, wheat germ agglutinin-ferritin (WGA-Fe) and concanaval in A-ferritin (Con A-Fe) binding to apical membranes of wounded and nonwounded rabbit corneal(More)
Pieces of glass coverslip coated with human fibronectin or human fibrinogen were implanted under one margin of a skin wound on adult newt (Notophthalmus viridescens) hind limbs. In contrast to uncoated glass or glass coated with nest serum, bovine serum or bovine serum albumin, glass treated with either fibronectin or fibrinogen supported considerable(More)
Nucleopore filters coated with various genetic types of collagen and certain collagen-derived peptides were implanted under one margin of a skin wound on adult Notophthalmus viridescens (newt) hind limbs. In contrast to their behaviour on untreated filters, epidermal cells migrated readily and to equal degrees on human type I, newt type I, bovine type II,(More)
The effect of cyclic AMP and related compounds on both in vivo and in vitro epidermal cell migration during wound closure in the adult newt was examined. Cyclic AMP (cAMP) and N6,O2'-dibutyryl cyclic AMP (db-cAMP) inhibited migration both in vivo and in vitro when used with equimolar concentrations of theophylline, an inhibitor of 3',5'-cyclic nucleotide(More)
Pieces of coverslip glass coated with various proteins were implanted under one edge of a fresh skin wound on adult newt hind limbs so that the implant served as wound bed for the migrating wound epithelium. Laminin, a protein that has been implicated as an epithelial-specific adhesin, was a moderately good migration substrate. Type-IV collagen, fibrinogen(More)
The effect of a synthetic peptide consisting of Arg-Gly-Asp-Ser (RGDS), the amino acid sequence representing the fibroblast attachment site in fibronectin (FN), was tested on migrating newt epidermal cells. In one approach, skin explants were placed on the bottom of plastic dishes coated with human FN, human fibrinogen (FGN), human serum spreading factor(More)
The effect of several solubilized monosaccharides on epidermal cell migration from skin explants of the adult newt was examined. The ability of epidermal cells to migrate on substrates coated with these same sugars or with wheat germ agglutinin (WGA) was also determined. Adding 0.05 M N-acetyl-glucosamine (GlcNAc) to the medium inhibited epidermal cell(More)