Dominique Vidal

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Burkholderia pseudomallei is the causative agent of melioidosis, an often fatal infection of humans and animals. The virulence of this pathogen is thought to depend on a number of secreted proteins, including the MprA metalloprotease. We observed that MprA is produced upon entry into the stationary phase, when the cell density is high, and this prompted us(More)
Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven(More)
The anthrax lethal toxin (LT) consists of two subunits, the protective antigen (PA) and the lethal factor (LF), and is essential for anthrax pathogenesis. Several recombinant antibodies directed against PA and intended for medical use have been obtained, but none against LF, despite the recommendations of anthrax experts. Here we describe an anti-LF(More)
Bacillus anthracis secretes two critical virulence factors, lethal toxin (LT) and edema toxin (ET). In this study, we show that murine bone marrow-derived dendritic cells (DC) infected with B. anthracis strains secreting ET exhibit a very different cytokine secretion pattern than DC infected with B. anthracis strains secreting LT, both toxins, or a(More)
Bacillus anthracis secretes 2 toxins: lethal toxin (LT) and edema toxin (ET). We investigated their role in the physiopathologic mechanisms of inhalational anthrax by evaluating murine lung dendritic cell (LDC) functions after infection with B. anthracis strains secreting LT, ET, or both or with a nontoxinogenic strain. Three lung cell populations gated on(More)
The effects of patulin and T-2 toxin were investigated on immunological responses of Balb/c mice. In vitro patulin had a stimulatory effect on splenocytes at lower concentration (1 nM to 10 nM) and strongly inhibited lymphocyte proliferation at higher concentrations (ID50 from 0.02 to 0.24 microM depending on mitogens). In the same experiments T-2 toxin was(More)
Burkholderia pseudomallei is the etiological agent of melioidosis, a potentially fatal disease occurring in man and animals. The aim of this study was to investigate the pathophysiological course of experimental melioidosis, and to identify the target organs, in an animal model. For this purpose SWISS mice were infected intraperitoneally with the virulent(More)
The present study was aimed at simplifying procedures to delineate species and identify isolates based on DNA-DNA reassociation. DNA macro-arrays harbouring genomic DNA of reference strains of several Burkholderia species were produced. Labelled genomic DNA, hybridized to such an array, allowed multiple relative pairwise comparisons. Based on the relative(More)
Ninety-four isolates of Yersinia pestis collected by the French army between 1964 and 1988 were evaluated for their susceptibilities to 24 antibiotics by the agar dilution method. All the isolates were susceptible to beta-lactam antibiotics including imipenem, to fluoroquinolones, aminoglycosides and to doxycycline. The most active compounds were(More)
The use of non specific immunomodulatory agents takes an important place in the aspecific host response to invading microorganisms. In this context, antimicrobial properties of royal jelly have been ascribed to organic acids (mainly 10 hydroxy-2-decenoic acid or 10-HDA) and proteins. We synthesized a derivative of 10-HDA, the(More)