Dominique Groleau

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Poly-β-hydroxybutyric acid (PHB) and similar bacterial polyesters are promising candidates for the development of environment-friendly, totally biodegradable plastics. The use of methanol, one of the cheapest noble substrates available, may help to reduce the cost of producing such bioplastics. As a first step, a culture collection of 118 putative(More)
  • D. Bourque, Y. Pomerleau, D. Groleau
  • 2004
Methylobacterium extorquens ATCC 55366 was successfully cultivated at very high cell densities in a fed-batch fermentation system using methanol as a sole carbon and energy source and a completely minimal culture medium for the production of poly-β-hydroxybutyrate (PHB). Cell biomass levels were between 100 g/l and 115 g/l (dry weight) and cells contained(More)
  • M. R. de Roubin, L. Bastien, S. -H. Shen, D. Groleau
  • 1991
Various physico-chemical parameters have been studied in order to improve the production of hepatitis B virus pre-S2 antigen (middle surface antigen) by the methylotrophic yeastHansenula polymorpha. Antigen production was done in two steps: first, production of cells on glycerol (Phase 1), followed by induction of antigen expression with methanol (Phase 2).(More)
The co-culture between Methylosinus sporium, a strictly aerobic methanotroph, and strictly anaerobic methanogens was studied in 5 L aerobic/anaerobic coupled granular sludge reactors under O2-limited conditions. The methanogenic bacteria maintained very good metabolic activities and were able to produce sufficient methane which serviced as substrate for(More)
  • V. M. Laube, D. Groleau, S. M. Martin
  • 2004
In the xylose fermentation of Bacillus polymyxa strain 9035, best 2,3-butanediol yields were obtained with 1.0 % yeast extract, 4–6 % xylose, shaking at 125 rpm and incubation at 30°C. Under these conditions, mannose, galactose, L-arabinose, cellobiose, starch and glucose were readily metabolized and yielded significant amounts of diol. Diol production from(More)
  • D. Groleau, V. M. Laube, S. M. Martin
  • 2005
The examination of the effect of N2, air and O2 on the glucose to 2,3-butanediol fermentation byBacillus polymyxa showed that N2 sparging resulted in best 2,3-butanediol production at low yeast extract concentration (0.5%, w/v) whereas aeration produced best results with high yeast extract levels (1.2%, w/v). However, under all atmospheric conditions,(More)
Geographic information systems have a primary role to play for sustainable urban development, as tools for collecting and analyzing environmental properties of buildings and urban spaces, either as a support for communication and public participation in urban planning. To this end, GIS should take into account the third dimension and they should be able to(More)
The opinions expressed in the abstracts are those of the authors and are not to be construed as the opinion of the publisher (Multimed Inc.), the organizers of the Third International Symposium on Hereditary Breast and Ovarian Cancer, or the Hereditary Breast and Ovarian Cancer Foundation. Although the publisher (Multimed Inc.) has made every effort to(More)
  • V. M. Laube, D. Groleau, S. M. Martin
  • 2004
The fermentation of glucose to 2,3-butanediol by Bacillus polymyxa was improved by increasing the amount of yeast extract in the culture medium. A level of 1.5% (w/v) resulted in optimal 2,3-butanediol production. A comparable fermentation could be achieved with 0.5% yeast extract if the phosphate level of the medium was increased from 0.0026 to 0.078 M and(More)
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