Dmitry S Loginov

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The gene egl3 of the filamentous fungus Penicillium canescens endo-1,4-β-glucanase, belonging to family 12 glycosyl hydrolases, was cloned and sequenced. The gene was expressed in P. canescens under the control of the strong promoter of gene bgaS, coding for β-galactosidase of this fungus, and efficient endoglucanase producer strains were obtained. The(More)
Genetic analysis of basidiomycete Trametes versicolor 1666 from the Komarov Botanical Institute (LEBIN) Collection has been carried out that verified the phylogenetic position of the strain and revealed the presence of a single laccase gene. A study of cultivation dynamics of basidiomycete showed a low level of laccase, peroxidase, and Mn-peroxidase(More)
Rate constants and activation parameters have been determined for the internal electron transfer from type 1 (T1) to type 3 (T3) copper ions in laccase from both the fungus Trametes hirsuta and the lacquer tree Rhus vernicifera, using the pulse radiolysis method. The rate constant at 298 K and the enthalpy and entropy of activation were 25 ± 1 s(-1), 39.7 ±(More)
A heterologous protein expression in the fungus Penicillium canescens is described for the first time. The fungal strains producing Trametes hirsuta 072 accase under control of a highly efficient promoter of the P. canescens gene bgaS has been constructed. These strains efficiently transcribe the T. hirsuta 072 laccase gene with a correct intron splicing.(More)
Plant xyloglucans play an important role in the processes of cell wall extension, determine their mechanical properties, thus affecting growth and morphology of individual cells and whole organs. Being one of the main components of hemicellulose, xyloglucans play a particular physiological role in woody plants. To study xyloglucan physiological role,(More)
Gene egl2 of secreted endo-(1–4)-β-glucanase of glycosyl hydrolase family 5 of the mycelial fungus Penicillium canescens was cloned. The gene was expressed in P. canescens under control of a strong promoter of the bgaS gene encoding β-galactosidase of P. canescens, and endoglucanase producing strains were obtained. Chromatographically purified recombinant(More)
Protein profiles of the basidiomycete Trametes hirsuta grown on standard medium without laccase as an inducer and on medium supplemented with CuSO4 were analyzed using a differential proteomics approach. Protocols developed for isolation and purification of extracellular and intracellular proteins of the mycelium allowed us to show extensive extraction of(More)
A comparative analysis of transcripts from the basidiomycota T. hirsuta grown with and without an inducer of the laccase biosynthesis was carried out. Methods of subtraction hybridization and massive parallel sequencing were used for this purpose. Unique transcripts encoded by genes that have a relatively high level of expression and belong to different(More)
Because of the wide application of laccases in different biotechnological processes and intense studies of the enzymes from different sources, the development of efficient techniques for monitoring laccase level is a task of significant importance. Enzyme-linked immunosorbent assay (ELISA) and Western blotting techniques were developed to control total(More)
The properties of transgenic aspen (Populus tremula) clones carrying the recombinant gene of xyloglucanase sp-Xeg from Penicillium canescens have been analyzed. Complex modifications were revealed both in the composition of the wood and in the plant phenotype. Biometric analysis showed that shoot dimensions increased by 24.8%, 25% and 26% in the(More)
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