Dmitry M. Grodzinsky

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Treatment of Allium cepa L. cellsuspension cultures with a biotic elicitor derived from the fungus Botrytis cinerea, resulted in phytoalexin synthesis. Two phytoalexins, 5-octylcyclopenta-1,3-dione and 5-hexyl-cyclopenta-1,3-dione, were accumulated in cultured onion cells. Removal of extracellular Ca2+ by the calcium chelator ethylene glycol(More)
Metabolites of a phytopathogenic fungus Botrytis cinerea Pers. were analyzed for the presence of biotic elicitors. Three groups of elicitors competent in inducing defense responses inAllium cepa cells were identified and partly purified. The recognition of the elicitor signal in onion cells was shown to elevate the concentration of reactive oxygen species(More)
Irradiation of barley seedlings by ultraviolet-B radiation influences the growth and development of the sexual elements of the spike, accelerating differentiation of the sporogenous tissue of the anther and the male gametophyte, a phenomenon which is accompanied by an increase in the asynchronicity of microgametogenesis and in heterogeny of the pollen(More)
Dose dependencies of growth and cytogenetic values have been built to determine that the critical level of root apical meristem damage induced by cute irradiation within the range from 2 to 20 Gy. Causal relationships between frequency of chromosome aberrations and death of tissue, organ, and organism have been analyzed. The critical level of damage in the(More)
The dose dependences of the frequency of chromosome aberrations in root meristems of pea germinants after their 48-h irradiation in a dose range from 4 to 8 Gy is nonlinear. The plateau of the curve indicates the activation of recovery processes. With the dose rise, the meristem size decreases, while the frequency of cellular inactivation, clustering(More)
Thirty years after the Chernobyl explosion we still lack information regarding the genetic effects of radionuclide contamination on the plant population. For example, are plants adapting to the low dose of chronic ionising irradiation and showing improved resistance to radiation damage? Are they coping with changing/increased pathogenicity of fungi and(More)
The relationship between changes in the methylation pattern of functionally different parts of DNA and the chromosomal aberration’s yield was studied under the conditions of UV-C irradiation fractionating. A combination of restriction analysis (HpaII, MspI, and MboI enzymes) with subsequent PCR (internal transcribed spacer ITS1 and ITS4 and inter simple(More)
This chapter consists of two main parts. The first deals with the phenomenon of biological fractionation of stable isotopes as a contribution to isotopic fractionation in geological processes. The second deals with biological roles of natural radioisotopes. We briefly address kinetic, thermodynamic, and magnetic isotope effects. Fractionation of stable(More)
Autoři sledovali současnĚ intensitu dýchání a intensitu fotosynthesy u listů cukrovky, neoddĚlených od rostliny. NĚkolik hodin před pokusem asimilovaly listy radioaktivní14CO2, načež byly umístĚny do normální listové komory k prď tokovému gazometrickému stanovení intensity fotosynthesy podle zmĚny koncentrace CO2 v procházejícím vzduchu. SoučasnĚ s(More)
Native PAGE was used to identify qualitative and quantitative changes in apoplastic enzymes extracted from bean leaves during a hypersensitive response (HR) resulting from inoculation with an avirulent race of P. s. pv. phaseolicola. A burst of apoplastic peroxidase (EC-POX) activity was identified 2 h after inoculation. There was an apparent increase in(More)