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Following peptide-bond formation, the mRNA:tRNA complex must be translocated within the ribosomal cavity before the next aminoacyl tRNA can be accommodated in the A site. Previous studies suggested that following peptide-bond formation and prior to EF-G recognition, the tRNAs occupy an intermediate (hybrid) state of binding where the acceptor ends of the(More)
The GTPase elongation factor (EF)-G is responsible for promoting the translocation of the messenger RNA-transfer RNA complex on the ribosome, thus opening up the A site for the next aminoacyl-tRNA. Chemical modification and cryo-EM studies have indicated that tRNAs can bind the ribosome in an alternative 'hybrid' state after peptidyl transfer and before(More)
Ribosomal variants carrying mutations in active site nucleotides are severely compromised in their ability to catalyze peptide bond formation (PT) with minimal aminoacyl tRNA substrates such as puromycin. However, catalysis of PT by these same ribosomes with intact aminoacyl tRNA substrates is uncompromised. These data suggest that these active site(More)
The fidelity of aminoacyl-tRNA selection by the ribosome depends on a conformational switch in the decoding center of the small ribosomal subunit induced by cognate but not by near-cognate aminoacyl-tRNA. The aminoglycosides paromomycin and streptomycin bind to the decoding center and induce related structural rearrangements that explain their observed(More)
Long-term exposure to dichlorvos (O,O-dimethyl-2,2-dichlorovinyl phosphate (DDVP), an organophosphate pesticide) is reported to exert neurotoxicity, i.e., generation of reactive oxygen species (ROS), oxidative damage, and neuronal cell death along with life- and health-span reduction in nontarget organisms including humans. However, studies on genetic(More)
The use of public cloud infrastructure for storing and processing large datasets raises new security concerns. Current solutions propose encrypting all data, and accessing it in plaintext only within secure hardware. Nonetheless, the distributed processing of large amounts of data still involves intensive encrypted communications between different(More)
Creative activities including arts are characteristic to humankind. Our understanding of creativity is limited, yet there is substantial research trying to mimic human creativity in artificial systems and in particular to produce systems that automatically evolve art appreciated by humans. We propose here to model human visual preference by a set of(More)
There is a significant body of empirical work on statistical de-anonymization attacks against databases containing micro-data about individuals, e.g., their preferences, movie ratings, or transaction data. Our goal is to analytically explain why such attacks work. Specifically , we analyze a variant of the Narayanan-Shmatikov algorithm that was used to(More)
A recent trend in ad hoc network routing is the reactive on-demand philosophy where routes are established only when required. Most of the protocols in this category, however, use single route and do not utilize multiple alternate paths. This paper proposes an analytical analysis of energy parameters, their usage and their evaluation in ad hoc networks. The(More)
Authorization logics allow concise specification of flexible access-control policies, and are the basis for logic-based access-control systems. In such systems, resource owners issue credentials to specify policies, and the consequences of these policies are derived using logical inference rules. Proofs in authorization logics can serve as capabilities for(More)